Intensive cultivation of the calenoid copepod, Bestiolina similis - PowerPoint PPT Presentation
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Intensive cultivation of the calenoid copepod, Bestiolina similis
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History of copepod aquaculture. High density captive production of finfish ornamentals. The advantage of copepods as a large scale food source for juvenile fish. ... – PowerPoint PPT presentation
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Title: Intensive cultivation of the calenoid copepod, Bestiolina similis
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Intensive cultivation of the calenoid copepod,
Bestiolina similis
- Quantifying the effects of food addition on
survival rates and reproductive success.
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Presentation outline
- Introduction
- Hypotheses
- Methods and Materials
- Results
- Conclusion
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Introduction
- Physiological development of the Bestiolina
similis
Egg 70x Early nauplius 40x Middle
Naupliu 40x Late Nauplius 25x Adult 10x
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History of copepod aquaculture
- High density captive production of finfish
ornamentals. - The advantage of copepods as a large scale food
source for juvenile fish. - Parameters of an efficient aquaculture system.
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Hypotheses
- Two biologic parameters crucial to an efficient
culturing system and which can be optimized - 1) Survival rates
- 2) Egg production
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Methods and Materials
- Phytoplankton cultures
- Cheatoceros
- Isochrisis
- Rhodomonas
- Concentration determined with use of
hemacytometer.
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Experimental setup
- Protocol for determining survival Rates
10-20 mL vials used in each
experimental trial to determine the effects of
varying additions of
phytoplankton cultures on
survival rates
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Protocol for determining survival Rates
- 1st) Sorting of copepods
- 2nd) Addition of phytplankton and calculation
of the concentration in vials - 3rd) Daily monitoring for number of deaths
- 4th) Daily water samples taken for nutrient
analysis - 5th) Determine survival rates
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Protocol for determining rate of egg production
- 1st) 2-1L cultures set up
- 2nd) Sorting of copepods
- 3rd) Calculation determining phytoplankton
volume addition - 4th) Timely isolation of copepods
- 5th) Egg count
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Results!!
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Note Rhdomonas has highest avg. of copepods
alive at 750 uL addition. Concentration is
2.85105 cells/mL
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Conclusions!!
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Survival rates
- Best Survival rates obtained from vials
containing 5.025104 cells/mL Rhodomonas - Is this absolute for all cultures?
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Egg laying rates
- Most eggs produced (30 eggs/female/day) and
highest average (15.9 eggs/female/day) occurred
in culture containing 2.775105 cells/mL
Isochrisis. - Is this absolute for all cultures?
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Discussion
- What did this research accomplish?
- What is the next step in copepod aquaculture?
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Achnowledgments
- I would personally like to thank
- My Mentor, Dr. Petra Lenz
- Program Director Dr. Micheal Cooney
- Laboratory Associate Ben Clauberg
- Support for this program was from NSF Sea
Grant 0243600.
