ERFPWorking Group Guidelines Cryopreservation Final report - PowerPoint PPT Presentation
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ERFPWorking Group Guidelines Cryopreservation Final report
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A practical guide, handbook, cookbook, checklist. Based on literature and experiences ... Integration of ex situ and in situ strategies and programmes ... – PowerPoint PPT presentation
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Title: ERFPWorking Group Guidelines Cryopreservation Final report
1
ERFP-Working Group Guidelines
Cryopreservation Final report
- Sipke J. Hiemstra
- Project co-ordinator
- 4 September 2004, NC-workshop Bled
2
- Objectives of project
- Results
- Evaluation of process and output
- Financial report
- Recommendations
3
Objectives (projectproposal november 2002)
- Development of guidelines for cryopreservation of
AnGR in Europe - Exchange of state of the art knowledge and
experiences with cryopreservation - Common approach, adressing same type of questions
and problems
4
Characteristics of guidelines cryopreservation
- To support individuals or organisations to start
or further develop cryopreservation programmes - Taking into account all major issues (eg legal,
veterinary) - To support decision making regarding the design
and operational choices - A practical guide, handbook, cookbook, checklist
- Based on literature and experiences
- European perspective
- Not for law-making, but can create awareness
among policymakers, stakeholders - Permanent draft
5
Final result 2004- Distributed April 2004-
Pdf available on internet
6
The process
- December 2002 Decision of SC-ERFP to approve
this project. - February 2003 Paris Workshop Cryopreservation
- April 2003 first steps outline of
guidelines-document - Two-day meeting of working group
- 12/13 June 2003, Lelystad, the Netherlands
- 13 participants from gt10 countries
- Summer 2003 Synthesis of meeting and additional
contributions of working group members resulted
in first draft - Progress report / presentation of first draft
guidelines at NC-workshop in Rome, 30 August
2003 - Sept 2003 March 2004 Chapter finalization and
preparation of final report - Sept 2004 Final report at ERFP workshop Bled
7
Contributors/participants
- Coralie Danchin-Burge (France), Barbara Gajda
(Poland), Gustavo Gandini (Italy), Eildert
Groeneveld (Germany), Ilma Grigaliunaite
(Lithuania), Sipke J. Hiemstra (the Netherlands),
Asko Maki-Tanila (Finland), Alfredo Martin
(Spain), Mihály Pásztor (Hungary), Flavia Pizzi
(Italy), Thomas A. Schmidt (Germany), Saffron
Townsend (United Kingdom), Morten Walløe Tvedt
(Norway), Jack Windig (the Netherlands), Henri
Woelders (the Netherlands)
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Outline
- Introduction
- Fundamental considerations
- Institutional setting and organisation
- Intake of genetic material
- Freezing and storage
- Use of genetic material
- Sanitary/veterinary requirements
- Documentation
- Legal issues
9
Fundamental considerations (2)
- Conservation of genetic diversity on long term
basis (CBD, ..) - Different perspectives, eg food security or
cultural heritage - Balance between ex situ and in situ
- Main objectives of cryopreservation (for future
use) - Back up for genetic problems in living
populations - Breed reconstruction
- Creation of new lines or breeds
- Back-up for future selection purposes
- Research
10
Institutional setting and organisation (3)
- Country specific strategies
- Territorial organisation regional national
European - Wide and active participation of stakeholders
- Public and private responsibilities
- Management
- Funding
- Cost benefit analysis
- Limited information about cryopreservation in
Europe
11
Intake (4)
- The type and amount of the material to be stored
is a function of the specific objectives of the
cryobank, of the budgets that are available and
of possible field constraints - Selection of species and breeds
- Include existing collections
- Many different criteria
- Selection within breeds (depends on objectives!)
- Sampling methods
- Number of donor individuals
- Type of genetic material
- Amount of material
- Time schedule
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Freezing and storage (5)
- Infrastructure (availability and choice)
- Freezing protocols
- Efficiency and efficacy
- By species
- By type of material
- References
13
Use (6)
- Property rights and rights of disposal
- Operational criteria and procedures
14
Sanitary considerations (7)
- How collection takes place
- How storage is organised
- Sanitary limitations for use
- EU regulations by species and type of material
- National regulations
- Collected material with approved (inter)national
sanitary status versus collected material with
no sanitary status
15
Documentation (8)
- Vital for future use
- Data on
- Donor animal
- Type of material
- Veterinarian status
- Legal status
- Location of samples in storage
- Software availabe
- Linkage to other (in situ) databases
16
Legal issues (9)
- Property rights and rights of disposal
- Material Acquisition Agreements
- Material Transfer Agreements
- ? Legal instruments should be developed further
in order to guarantee long time viability of a
cryopreservation program
17
Evaluation of the output
- Comprehensive chapters with conclusive summary
- At least useful checklist
- All relevant issues together in one document
- Differences between chapters
- New issues
- Based on extensive list of scientific research
- Based on experiences
- Valuable set of references by chapter
- Document is not finished, (regular) improvement
necessary - Feed back ?
18
Evalution of the process
- Exchange of state of the art knowledge and
experiences resulted in a useful guide and can be
distributed and discussed further - Network building in Europe and abroad
- Exploration of new major issues
- Valuable interaction of individuals with
different background, expertise or experiences - Much can be done with some seed money, but
input of experts is limiting factor - Ownership of the output
- Limited information about situation in all
European countries - Follow up is recommended
19
Financial report
- Budget 20.000 Euro
- Expenses -/- 11.288 Euro
- (4.921 Euro travel expenses) (3.256 Euro
expenses Lelystad-meeting) (incl hotel, excl
travel) (3.111 Euro expenses
publication/distribution) - Balance 8.712 Euro
20
Conclusions and Recommendations
- Working group made first attempt to assemble all
relevant issues regarding cryopreservation - Useful guidelines but animal sector deserves
further development of some major issues - Co-operation between ERFP-network, EAAP-working
group and other expert/interest-groups is very
fruitful - Last version available on ERFP website (pdf)
- Collaboration with FAO and global network of NCs
- More detailed information about situations in all
European countries is necessary - Integration of ex situ and in situ strategies and
programmes
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- THANK YOU
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