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Integrated NanoparticleBiomolecule Hybrid Systems: Synthesis, Properties, and Applications
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with biomolecules yields new. facets of bioelectronics to open. new horizons of nanobioelectronics. Herein, we aim to review the. synthesis and properties of ... – PowerPoint PPT presentation
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Title: Integrated NanoparticleBiomolecule Hybrid Systems: Synthesis, Properties, and Applications
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Integrated NanoparticleBiomolecule Hybrid
SystemsSynthesis, Properties, and Applications
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- C2007
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From the contents
- Introduction
- Synthesis and properties of Biomolecule-Functional
ized Nanoparticles - Bimolecule-Functionalized Nanoparticles for
Controlled Chemical Reactivity - The Aggregation of Bimolecule-Functionalized
Nanoparticles - Assembly of Bimolecule-Nanoparticle Architectures
on Surfaces - Bimolecule-Based Nanocircuitry
- Conclusions
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Nanobiotechnology
- Combination of nanoobjects,
- nanotools, and nanotemplates
- with biomolecules yields new
- facets of bioelectronics to open
- new horizons of nanobioelectronics.
- Herein, we aim to review the
- synthesis and properties of
- biomoleculenanoparticle/nanorod
- hybrid systems as well as
- the organization of these systems
- as functional devices.
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Functionalized of Nanoparticles with Biomolecules
Through Electrostatic Adsorption
- The simple adsorption of
- biomolecules on NPs has
- frequently been performed
- and studied for biomolecules,
- which range from low-
- molecular-weight organic
- substances e.g. vitamin C)
- to large (protein/enzyme
- molecules.) NPs that are stabilized
- by anionic ligands such as
- carboxylic acid derivatives (citrate,
- tartrate,lipoicacid), theadsorption
- of positively charged proteins
- originates from electrostatic
- Interactions.
- .
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CdS NP-capped mesoporous silica nanosphere
(MSN)-basedsystem for the controlled delivery of
drugs andneurotransmitters
- Functionalized CdS NPs were
- used to entrap drugs or
- neurotransmitters in the
- channels (average diameter
- 2.3 nm) of MCM-41-type
- mesoporous silica nanospheres
- (MSNs) in a configuration
- that enabled the controlled
- releaseof the entrapped
- Substances.
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Receptor-Induced Aggregation of
Guest-FunctionalizedNanoparticles
- Au NPs functionalized with
- adsorbed biotin units and
- then cross-linked with SAv
- units have been shown to
- yield aggregates of Au NPs
- with the biotinSAv recognition
- pairs between the individual NPs .
- A similar architecture can be built
- by reversing the steps SAv was
- interacted with the biotin
- disulfide derivative 13 to produce
- a complex, which was then
- treated with Au NPs. In both
- cases, fast, spontaneous
- aggregation of the Au NPs was
- observed which resulted in a
- nonordered network of particles.
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Nucleic AcidNanoparticle Architectures on
Surfaces
- Nucleic acids can serve as
- templates that bind DNA-
- functionalized nanoparticles
- at complementary segments.
- When DNA templates are
- fixed at surface of a solid
- support, the resulting
- assemblies of NPs can yield
- a pattern that is dependent
- on either the shape
- produced by the DNA
- template itself or on the
- pattern produced upon its
- Immobilization.
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Biomolecule-Functionalized Nanoparticles for
Controlling DNA Reactivity
- The absorbance of a solution of the Au
- NPDNA molecular beacon conjugate 2 at
- 260 nm as a function of the time in which
- the electromagnetic field is switched on
- and off. The increase in the absorbance
- reflects the denaturation of the DNA
- Doublehelixupon local heating, whereas
- the decrease in the absorbance
- corresponds to the DNA rehybridization
- When the electromagnetic field is
- switched off. The switching between two
- distinct states is fully reversible. A
- control experiment revealed that the
- DNA molecular beacon, 3, which lacks
- the Au nanoparticle, is not affected by
- the electromagnetic field (Figure 5A,
- curve b). Although inductive heating has
- already been applied to macroscopic
- samples as well as to the treatment of
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Composite Assemblies of Nucleic Acids, Proteins,
and Nanoparticles
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- Multilayers of nanoparticles can be
- assembled on solid supports by utilizing
- DNA complementarity. For this purpose, a
- glass surface was functionalized with a
- monolayer of an oligonucleotide, 46, and
- then the surface was treated with an
- oligonucleotide, 47, which was composed of
- two domainsone domain was
- complementary to 46, whereas the second
- provided complementarity for 48. Au
- nanoparticles (13 nm) that were
- functionalized with oligonucleotide 48were
- then added to yield a monolayer of ds-DNA
- (ds-47/48) attached to the Au NPs.
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- Efficient methods for the preparation
- of semiconductor NPs (e.g. CdS, CdSe,
- PbS, ZnS) and their functionalization
- with biomolecules were recently
- developed. These NPs were applied as
- labels of biomaterials in biorecognition
- processes such as DNA sensing. For
- instance, CdS semiconductor NPs that
- were modified with nucleic acids were
- employed as tags for the detection of
- hybridization events of DNA.
- Dissolution of the CdS NPs (in the
- presence of 1m HNO3), followed by
- the electrochemical reduction of the
- Cd2 to Cd0, which accumulates
- on the electrode, and theremoval
- of the generated Cd0 (as Cd2)
- provided the electricalsignal for the
- DNA analysis.
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