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ResultsPhenotypic profile of shortterm cell lines
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CD8 T cells were stimulated in 2 out of the 3 ... Nester, Eugene W., C. Evans Roberts, Nancy N. Pearsall, Denise G. Anderson, and ... Steinman, Lawrence. 1996. ... – PowerPoint PPT presentation
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Title: ResultsPhenotypic profile of shortterm cell lines
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Results-Phenotypic profile of short-term cell
lines
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Conclusion-Figure 3.
- CD8 T cells were stimulated in 2 out of the 3
patients - Phenotype of responding cells differed depending
on the vaccine clones used - Overall cytokine response was seen from TCR-ab
- All the clones stimulated Natural Killer cells
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Rationale-Figure 4.
- To determine the type of immune response that is
generated by T cell vaccination - Non-stimulated and stimulated PBMC cultures were
studied all in vitro
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Figure 4.-Cytokine Production Profile
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Conclusion-Figure 4.
- Controls PBMC cultures did not produce IL-4
- Some control cases showed low levels of IFN-g
- IFN-g and TNF-a production was observed in most
cultures - IFN-g production increased in most patients after
the 3rd vaccination
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Rationale-Figure 5.
- To study the relative contributions of CD4,
CD8, and gd T cells to cytokine production after
vaccination - Certain T cells were removed from the PBMC
- Controls were PBMC containing all T cells
- Depleted cell mixtures were irradiated in vitro
with vaccine cells - Results were obtained using flowcytometry
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Figure 5.-Proliferation and cytokine production
of depleted PBMC
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Conclusion-Figure 5.
- CD4 cells most often reduced proliferation
- Depletion of CD8 T cells did not greatly alter
proliferation - In some cases, gd T cell depletion raised
proliferative activity - Depletion of gd T cells did not have a major
effect on cytokine production - The major effector of cytokine production was
CD4 T cells
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Conclusion
- Precise mechanisms by which T cell vaccination
ameliorates autoimmune disease are still unclear - The majority of patients the proliferative
response was maximal after the 2nd and 3rd
vaccinations - For long term T cell response, SI units greater
than 2 were observed in at least one clones for
all patients (See Figure 2) indicating there may
be memory antigen-specific lymphocytes
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Conclusion Contd
- From Figure 3., it is concluded that the
phenotypic characteristics obtained after
stimulation with vaccine cell lines demonstrate
that CD4/- and CD8/- cells respond to the
vaccine - Figure 4., reinerates the fact that cytokine
production increased mainly after the 3rd
vaccination - Depletion of CD4 T cells led to a substanial
reduction in proliferative response indictaing
CD4 T cells role to vaccine responses in vitro
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References
- Hermans, Guy, Ulrike Denzer, Ansgar Lohse, Jef
Raus, and Piet Stinissen. 1999. Cellular and
Humoral Immune Responses Against Autoreactive T
cells in Multiple Sclerosis Patients After T cell
Vaccination. Journal of Autoimmunity 13
233-246. - Nester, Eugene W., C. Evans Roberts, Nancy N.
Pearsall, Denise G. Anderson, and Martha T.
Nester. 1998. Microbiology A Human
Perspective. 2nd edition. Boston WCB
McGraw-Hill. 367-370pp. - Pouly, Sandrine and Jack P. Antel. 1999.
Multiple Sclerosis and Central Nervous System
Demyelination. Journal of Autoimmunity
13297-306. - Steinman, Lawrence. 1996. Multiple Sclerosis A
Coordinated Immunological Attack against Myelin
in the Central Nervous System. Nature 365
642-644. - http//mail.med.upenn.edu/hessd/Lesson2.html
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