TATER TOTSOR NOT - PowerPoint PPT Presentation
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Title: TATER TOTSOR NOT
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TATER TOTSOR NOT?
Linda Knewstub 2006
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- This ppt can be used to help teachers introduce
the Tater tots (tissue culture) lab - It can also be used to help guide follow up
discussion.
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Curriculum topics
- Living vs Non Living
- Traits of Living Things (Life Functions)
- Requirements of Photo. Resp. (Cell energy)
- Scientific Method Experimental Design
- Cell division mitosis, asexual reprod,
vegetative propagation, stem cells
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Living ?
YES
NO
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Living or Non Living?
Must Show All Traits of Living things
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Hypothesis
IF..THEN..
- Measure increased length
- Grow new parts, leaves, potatoes, etc
- Use up food
- Stay alive
- Growth
- Development
- Obtain Use energy
- Maintain Homeostasis
- Reproduce
- Respond to environment
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PROCEDURE
- Wash hands,etc.
- Spray down desk with alcohol
- Sterilize tools then set to dry
- Set up work station
- Cut node from top, middle, bottom
- Place on petri plate and record position (Cover
at 450 angle) or culture tube - Seal w/ micropore tape and label
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PROCEDURE
- 2. Spray down work area
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PROCEDURE
- 2. Spray down work area
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PROCEDURE
- 4. Set up work area
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PROCEDURE
- 5. Sterilize tools then blot them dry
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PROCEDURE
- Cut node from top, middle, bottom
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PROCEDURE
- 7. Place on petri plate and record position
(Cover at 450 angle)
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PROCEDURE
- Or
- 7. Place in culture tube and record position
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Put half in cold/darkhalf in light /warm
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Make observations
- What type of quantitative data might you collect?
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Make observations
- What type of qualitative data might you collect?
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Follow up Discussion
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SUMMARY
- Write a statement about each of the following
- of Tubers
- amount of Stem/leaf growth
- Amount of contamination
- Position on the plant
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Curriculum Topics
- Living vs Non Living
- Traits of Living Things (Life Functions)
- Scientific Method Experimental Design
- Requirements of Photo. Resp. (Cell energy)
- Cell division mitosis, asexual reprod,
vegetative propagation, stem cells
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What did we get out of this ?
- Realize that what looks dead may not be dead
(Curriculum topic1) - Cells (not just organisms) are the basic unit of
function (growth, development, reproduction,
metabolism, response) (topics 1,23)
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What did we get out of this ?
- Tissue culture and Sterile technique (topic 3)
- Analyze real data and (topic 3)
- Suggest further experiments that isolate and test
for single variables (topic 3)
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What did we get out of this ?
- That light is a requirement for photosynthesis
and chlorophyll production (topic 4) - That tubers are a form of asexual reproduction
and vegetative propagation (topic 5) - Recognize that undifferentiated cells can
differentiate into specialized plant parts - Realize that environment can influence the
expression of traits
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