1 kb ladder

1
Control Gel
pCAT BamH1-HindIII partial digest
pCAT BamH1-HindIII-EcoR1
Note the partial digest has the linearized
plasmid, the plasmid backbone and the CAT fragment
pCAT BamH1-HindIII
pBS BamH1-HindIII
pCAT BamH1
pCAT HindIII
pBS BamH1
pCAT uncut
pBS HindIII
1 kb ladder
pBS uncut
CAT Plasmid backbone
3.0 kb
CAT fragment
2.0 kb
2
Tuesday lab gel 1
1 kb ladder
Emily/Rosario
DJ
Melanie/Laura
4
1
2
3
3
1
1
4
2
2
3.0 kb
2.0 kb
Vector fragment
CAT fragment
3
Tuesday Lab Gel 2
Susan/ Aleks/ Laura
Elizabeth/ Vidi/Pavani
1 kb ladder
Adam/Meredith
Biff/Althea
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
Vector fragment
3.0 kb
2.0 kb
CAT fragment
4
Wednesday Lab Gel 1
1-4 EB/MB BS cut, CAT cut, BS uncut, CAT
uncut 5-8 ASC/DD BS cut, BS uncut, CAT cut, CAT
uncut 9-13 SQ/TT BS uncut, BS cut, CAT uncut,
empty well, CAT cut 14-17 DS/BAM BS cut, BS
uncut, CAT cut, CAT uncut Marker
EB/MB
ASC/DD
SQ/TT
DS/BAM
1
1 kb ladder
2
3
4
5
6
7
8
9
10
11 12
13
14
15
16
17
Vector fragment
3.0 kb
2.0 kb
CAT fragment
5
Wednesday Lab Gel 2
1-4 DCP/WR CAT cut, CAT uncut, BS cut, BS
uncut 5-8 SJK/FMM BS cut, BS uncut, CAT cut, CAT
uncut 9-12 AW/JG/AF BS uncut, BS cut, CAT uncut,
CAT cut 13-16 AW/AB CAT cut, CAT uncut, BS cut,
BS uncut
DCP/WR
3
1 kb ladder
SJK/FMM
AW/AB
AW/JG/AF
1
2
5
6
7
8
4
9
10
11
13
14
15
16
12
Vector fragment
3.0 kb
2.0 kb
CAT fragment