ADVANCED STUDIES IN MATERIALS SCIENCES: BIOSENSORS FROM SEMICONDUCTORS

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ADVANCED STUDIES IN MATERIALS SCIENCES
BIOSENSORS FROM SEMICONDUCTORS

• Dr. Christoph E. Nebel
• Diamond Research Center/AIST/Tsukuba/Japan
• Email Christoph.nebel_at_aist.go.jp
• Tel [phone]

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I. Introduction Biosensor Principle
Biosensor Principle
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Bio-Detection Techniques
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Transducer Figure of Merits
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Carbon-Nanotubes
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Carbon nano-Tube Sensing ArrayPotentiometric
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Amperometric Sensing
DNA intercalation
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Amperometric Sensing
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2) Double stranded (ds) DNA
1) Single-stranded (ss) DNA
Redox-Molecules are repelled by Coulomb Force
Redox-Molecules diffuse into the ss-DNA film,
ds
ss
Generation of Redox- Current
No Redox- Current
ds
ss
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Aims

• This course covers the
• Principles,
• Technologies,
• Methods
• of biosensing, using semiconductors like
• Silicon,
• Diamond,
• Carbon Nano-Tubes
• others
• which are of interest for biosensing and which
  can be
• miniaturized for single molecule detection.

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The objective of this course is to develop

• basic understanding of sensing principles
  (physics and chemistry),
• biosensor realization methods (technology)
• typical fields of application.
• It will provide students detailed knowledge about
  methods and procedures used for sensing, design,
  and application. Upon successful completion of
  this course, students are expected to be able to
  explain biosensing principles and transducing
  techniques, design and construct of semiconductor
  based biosensors.

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LECTURE SYLLABUS

• Dates
• 10.12.2007 (canceled)
• 17.12.2007
• 07.01.2008 (canceled)
• 21.01.2008 Overview of Biosensors
• 28.01.2008
• 04.02008
• 11.02. 2008 Holiday
• 18.02.2008
• 25.02.2008

1. Introduction of Lecturer and Lecture
2. Electrochemical Biosensors (Amperometric
Detection) Electrochemical principles Transducer
Materials Amperometric biosensors and charge
transfer pathways for DNA sensing Multi-array
(ultra-miro-, ultra-nano-electrode arrays)
sensing and their potential for cancer detection
Engineering electrochemical biosensors for
single molecule detection 3. Transistor Based
Biosensing (Potentiometric Detection) Detection
principles Materials and nano-technology (from
single electron transistors to carbon
nano-tubes) Detection and sensing of neuronal
networks
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COURSEWORK

• The coursework will involve a laboratory session
  at DRC/IAST Tsukuba
• (if agreed to by students)
• illustrating the realization of diamond based DNA
  sensors at
• AIST /DRC.

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OBJECTIVES

• On completion of the module students should
• Be able to understand the physical and chemical
  principles applied for electronic bio-sensing
  using semiconducting transducers.
• Understand the principles of linking cell
  components like DNA to solids, of electronic
  signal generation by biological activities, of
  sensing and of detection.
• Understand the basic configuration and
  distinction among biosensor systems
• Learn the technical advantages and limits of such
  devices and their future fields of applications.

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REFERENCES

• 1) Biosensors, Fundamentals and Applications,
  Edited by A.P.F. Turner, I. Karube, G.S. Wilson,
  Oxford Science Publication, Oxford University
  Press, 1987
• 2) Semiconductor Sensors, Edited by S.M. Sze,
  John Wiley Sons, Inc. New York,1994
• 3) To each selected topic, copies of published
  papers in well recognized journals like Nature
  and Science will be distributed by the
  lecturer.

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Source of Information

• Homepage
• http//unit.aist.go.jp/dia-rc/dia-surf/index.htm
• Email christoph.nebel_at_aist.go.jp