Evaluation of precorecore promoter sequences in hepatitis B virus and HIVcoinfected patients in a 3y

1
Evaluation of precore/core promoter sequences in
hepatitis B virus and HIV-coinfected patients in
a 3-year follow-up study
WEPDB206

• Cassino L., Laufer N., Moretti F., Bouzas MB.,
  Cahn P., Salomon H., Quarleri J.
• Centro Nacional de Referencia para el SIDA, UBA
• Hospital Fernández, Servicio de Infectología
• Hospital Muñiz, Unidad de Virología

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• HBV-HIV coinfection

• The HBV genotype is considered a factor that
  influences clinical and virological features of
  HIV-infected individuals.
• In Argentina, the genotype F is prevalent among
  blood donors. In coinfected patients we reported
  the prevalence of genotype A.
• Genomic variations in regulatory regions of HBV
  might have an impact on viral replication and
  drug resistance

Objective To study the impact of genomic
variations on HBV Pc/C region in HBV-HIV
coinfected patients.

• Methods
• 19 HIV-HBV-coinfected patients were evaluated
  for 3 years.
• 12 HBeAg 10 A, 1D, 1F
  (based on S gene).
• 7 HBeAg- 6 A, 1F.

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Among 19 isolates, 13 did not show BCP/Pc
variations during the follow up study.
Results

• 6 isolates exhibited variations

4
Conclusions

• HBV isolates characterized as Genotype A from
  HIV-coinfected patients, rarely exhibits BCP/Pc
  variations, regardless of viral fitness and HBeAg
  expression.
• The polyphyletic quasispecies distribution into
  each HBV-Genotype A isolate was maintained during
  the 3year follow-up study suggesting a
  non-ancestral relationship despite immune and
  drug pressure.
• The prevalence of mutations in regulatory
  sequences among HBV genotypes could play a role
  in the different progression observed in
  HIV-coinfected patients.