1. intermediate proteins.

1
????? ??????
?? ??????
1. ????? ????? ?????? ?????? intermediate
proteins)).
2. ????? profile-profile
3. ????? ????
4. HMM
2
Pfam
Protein families database of alignments and
HMMs http//www.sanger.ac.uk/Software/Pfam/

• ?- 3000 ?????? ???? 70 ?-SwissProt.
• ???? ????? ??????? ???????
• ???? ??? ????? ??? ?????? ????? ?????? (MSA)
  ??????? ?????. ??? ?????? ????? ????? ?? ????
  ???????.
• ???? ???? ????, ??????????? ????????? ??.

??? ???? ???? ???? ?- PSI-BLAST ?
3
FFAS03 Fold Function Assignment System
http//ffas.ljcrf.edu/ffas-cgi/cgi/ffas.pl PSI-BLA
ST on steroids

• ?? ?????? ?????? ?- PSI-BLAST.
• ??????? ?????? PSI-BLAST ?????? ????????? ???????
  ?- Pfam, SCOP, COG ??????.

?? ???? ???? ?????? ???? ?? ?????? ???????
4
CLUSTAL_X Thompson et al. Nucleic Acids Research
254876 Thompson et al. Nucleic Acids Research
224673

• ????? ????? ???? (Multiple Sequence Alignment)
• ????? ?????? ?????? ?? ??? ???? ??????????
• ?? ????? ?????? ?????? ?? ??? ????? ??????
  ??????? ??????.
• ??????? ??????? ???? ?? ???????? ??????.
• ?? ??? ???? ???? ????? ?????? ?????? ? ?????? ???
  ???????.
• ????? ?????? ????? ????? ?????? ????? ???
• ??????? ??????? ?????? ??????? ?????, ?????
  ?????? ?????? ??????? ????? ???? ?? ??????.
• ??? ????? ?????? ??????? ??? ??? ??? ?? ?????
  ?????? ???? ????? ?? ?????? ?????.
• ?????? ?????? ???????? ????? ???? ??????? ???????
  ???? ???? ?? ??????, ???? ????? ????? ??? ?? ???
  ??????.

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?? ?????? ??????? ????? ??. ????? ??? ????
???????? T-cofee ?- MUSCLE ?? ????? ????
10
????? ????? ???????? ??? ??????? ????? ??????
?????? ????? ?? ??? ???????PSI-BLAST