imunohistoqimiis diagnostikuri SesaZleblobebi prof. g. burkaZe

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imunohistoqimiis diagnostikuri SesaZleblobebi
prof. g. burkaZe
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• imunohistoqimia
• radioaqtiuri moniSvna - radioimunohistoqimia
• enzimiT moniSvna - enzimoimunohistoqimia
• fluorescentuli nivTierebiT moniSvna -
  imunofluorescencia

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• imunohistoqimiis saxeebi da vizualizaciis
  sistemebi
• 1. kunsis pirdapiri da arapirdapiri meTodebi
• peroqsidaza-antiperoqsidazuli (PAP) meTodi
• LSAB (Labelled Streptavidin-Biotin) meTodi
• ABC (avidin-biotin complex) meTodi
• EnVision

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LSAB (Labelled Streptavidin-Biotin)
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Immunophenotypes of major groups of neoplasms
www.dako.com
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Breast carcinoma, ER
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Breast carcinoma, PgR
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Breast carcinoma, p53
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Breast carcinoma, Ki-67
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• HER2/neu gene is a proto-oncogene located on
  17q21 and encodes a transmembrane glycoprotein
  with intrinsic TK activity that regulates various
  important aspects of the growth and
  differentiation of cells

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HER2 amplification leads toHER2 overexpression
HER2 overexpression leads to tumour proliferation
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Significance of HER2 positivity

• Prognostic value
• association with aggressive disease and poor
  clinical outcome
• Predictive value
• identification of patients likely to benefit from
  Herceptin
• prediction of relative sensitivity to
  anthracycline- and taxane-based regimens
• indication of decreased sensitivity to tamoxifen
  and CMF

Hanna W, Gelmon K. Current Oncology 20029(Suppl.
1)S2S17 Ross JS, et al. Oncologist 2003830725
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IHC scoring semi-quantitativeinterpretation of
HER2 expression
1 (negative)
0 (negative)
2 (equivocal)
3 (positive)
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Dual-colour FISH scoring
No amplification HER2 negative
Amplification HER2 positive
Increased HER2 genecopy number
Normal HER2 genecopy number
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HER2 testing algorithm
Patient tumour sample
FISH
IHC


2
3
1
0
Herceptin therapy
Herceptin therapy
FISH


Herceptin therapy
Bilous M, et al. Mod Pathol 20031617382
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Prostate sclerosing adenosis
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Immunoreactivity for S-100 protein further
supports myoepithelial differentiation of basal
cells in sclerosing adenosis
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PTEN, endometrial intraepithelial neoplasia
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CA-125, serous cystadenoma of ovary
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Calretinin, mesothelioma
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limfoiduri infiltracia kuWSi, HE
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kuWis maltoma, HE
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kuWis maltoma, Bcl-10, IHC
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kuWis maltoma, p53, IHC
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kuWis stromuli simsivne, HE
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kuWis stromuli simsivne, CD117, IHC
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Follicular Center Cell Lymphoma
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Follicular Center Cell Lymphoma
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Follicular Center Cell Lymphoma, BCL-2
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Follicular hyperplasia, BCL-2
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msxvili nawlavis adenomatozuri polipi, HE
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msxvili nawlavis adenokarcinoma, HE
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nawlavis adenokarcinoma, p53, IHC
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RviZlis adenoma, HE
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hepatoceluluri karcinoma, HE
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hepatocitebis specifikuri antigeni, IHC
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pirveladi glomerulonefriti 1. mwvave difuzuri
moproliferacie glomerulonefriti poststreptokokuli
arapoststreptokokuli 2. mwvave moprogrese
glomerulonefriti (naxevarmTvariseburi) 3.
membranuli glomerulonefriti 4. lipoiduri nefrozi
(minimaluri cvlilebebis daavadeba) 5. fokaluri
segmenturi glomerulonefriti 6. membrano-proliferac
iuli glomerulonefriti 7. IgA nefropaTia 8.
fokaluri moproliferacie glomerulonefriti 9.
qronikuli glomerulonefriti
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meoradi glomerulonefriti SLE Saqriani
diabeti amiloidozi gudpasCeris sindromi kvanZovani
polarteriti vegeneris granulomatozi henox-Sonlain
is daavadeba baqteriuli endokarditi alportis
sindromi fabris daavadeba
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mwvave proliferaciuli glomerulonefriti, HE
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Mmwvave difuzuri proliferaciuli
glomerulo-nefriti, sistemuri wiTeli mglura, HE
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Mmwvave moprogrese glomerulonefriti, HE
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Mmwvave moprogrese glomerulonefriti, CD68, IHC
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Mmwvave manekrozebeli eqstrakapilaruli
glomerulonefriti, masonis triqromi
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Mmwvave moprogrese glomerulonefriti, CD3, IHC
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Mmwvave difuzuri proliferaciuli
glomerulo-nefriti, fibrini, imunofluorescencia
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Mmwvave difuzuri proliferaciuli
glomerulo-nefriti, IgG, imunofluorescencia
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Mmwvave difuzuri proliferaciuli
glomerulo-nefriti, IgA, imunofluorescencia
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Mmwvave difuzuri proliferaciuli
glomerulo-nefriti, C3, imunofluorescencia
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Mmwvave difuzuri proliferaciuli
glomerulo-nefriti, C1q, imunofluorescencia
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