SERS of roughened gold electrodes.

1

• SERS of roughened gold electrodes.
• Separation of gold particles with different shape
  by electrophoresis.
• Prepare gold prism film.
• Yan, Wei group, 06/04/04

2
Prepare roughened gold electrode Gold foils were
treated with propene torch and sonicated for
10min. Electrochemistry treatment for 30
sweeps Solution 0.1M KCl EI-300mV DI1.3s Scan50
0mV/s EI1.2V DI30s SERS was taken by NIRM(
784.45nm) , ?40 objective, 10s
3
In order to get reasonable results, I make sure
that the SERS of the freshly prepared gold
electrode was like this before further treatment.
4
Roughened gold electrode soaked in Pure
Morpholine
5
Roughened gold electrode soaked in pure CS2 for
10 min, and then soaked 0.1M morpholine THF
solution and pure morpholine successively. Major
peaks at 453 and 840.
6
Roughened gold electrode soaked in Pure CS2 for
10 min, and then soaked 0.1M morpholine THF
solution. When the sample was washed by D.I.
Water, Peak 840 were split to two. When the
sample was further soaked in 10mM HClO4, the
peak at 853 grow a little.
7
Roughened gold electrode soaked in pure Piperidine
8
Roughened gold electrode soaked in Pure CS2 for
10 min, and then soaked 1mM piperidine THF
solution. Major peaks at 444.
9
Roughened gold electrode soaked in Pure CS2 for
10 min, and then soaked 1mM piperidine THF
solution. When the sample was washed by D.I.
Water, the spectrum is nearly the same. When the
sample was further soaked in 10mM HClO4, the
peak at 444 split to two, while a new peak at
946 appeared.
10
Roughened gold electrode soaked in Pure CS2 for
10 min, and then soaked 1?M didecylamine THF
solution.
11
Roughened gold electrode soaked in Pure CS2 for
10 min, and then soaked 1mM didecylamine THF
solution.
12
Roughened gold electrode soaked in Pure CS2 for
10 min, and then soaked in 1?M didecylamine THF
solution. When the sample was washed by D.I.
Water, and further soaked in 10mM HClO4, the
spectra were nearly the same.
13

• I made large amount of gold triangle this week.
• Stabilize gold prism particles
• 10mL Au prism particle sol(40nm) was treated with
  MB-3 for overnight.
• added with 4mg dipotassium bis(p-sulfonatophenyl)
  phenylphosphane dihydrate for 10 hours.
• Centrifugated 2 at 5000RPM for 10min, remove the
  supernatant and redispersed the particles in 5ml
  water.

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Electrophoresis 0.5 agarose, TBE buffer, voltage
70V As lower concentration of agarose was used,
the gold prism moved faster, but the separation
is not as good.
15

• Prepare gold prism film
• 1ml Gold prism sol was treated with MB-3 for
  overnight.
• Add 1.5ml C11(1mM), and votex.
• Add 1ml Toluene.
• Washed 3 by Toluene for 3 times.
• Dropped on the surface of water, a film can be
  seen.
• Made TEM sample.

16
25?l 0.05M Na3citrate and 16?l 0.1M NaCl was
added before adding C11.
17

• Next week
• As Boemsoek was busy this week, we will prepare
  the gold prism film next week.
• Try to separate the gold particles with different
  shape with electrophoresis.
• SERS of roughened gold electrodes.