Enzyme Mechanism

1
Enzyme Mechanism

• Michaelis constant, KM
• Strength of attraction between enzyme and
  substrate
• Pick up oranges with left hand and peel them with
  right hand
• Maximum velocity, Vmax
• Maximum speed of the reaction
• Put orange down and pick up a new one

2
Enzyme Regulation
0

• Inhibition (Reversible and Irreversible)
• Competitive
• Compete for active site, affects KM
• Add plastic balls to the bin of oranges
• Non-competitive
• Alters enzyme at site other than active
• Changes Vmax
• Put glove on right hand, slows peeling rate

3

• Allosteric enzymes binding of substrate to one
  domain affects the binding to other parts of a
  quaternary structure
• Positive and negative effectors
• Feedback and feedforward control
• Zymogens
• pepsinogen pepsin
• chymotrypsinogen chymotrypsin