Laboratory Facilities and Safety

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Laboratory Facilities and Safety

• Paul B. Kimsey, PhD.
• Rebecca Sciulli, M. Sc, M.T.

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Biosafety in Microbiological and Biomedical
Laboratories

• BMBL 4th Edition
• CDC/NIH
• Canadian Laboratory Biosafety Guidelines
• WHO Biosafety Guidelines

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Principles of Biosafety

• Laboraotry Practice and Techniques
• Safety Equipment
• Facility Design and Construction
• Biosafety Levels

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Laboratory Practice and Technique

• Adherence to standard micro practices and
  techniques
• Develop Biosafety Manual
• Risk Assesment

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Safety Equipment

• Primary Barriers
• Minimize exposure
• BSCs
• Class I, II, III
• Gloves, coats, respirators
• Safety centrifuge cup

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Biosafety Practices Plan

• Written procedures on the proper way to
  don/remove PPE
• Entering/leaving isolation suites
• Protocols on the use of Biosafety Cabinets
• Decontamination protocols
• Spills
• Surface
• Instruments

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Facility Design and Construction

• Secondary Barriers
• Design and Construction
• Risk Assessment
• Public Access
• Autoclaves
• Handwashing

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Laboratory Biosafety Level Criteria

• Four Levels 1, 2, 3, 4,
• Known Agents by category
• Unknown Agents
• Risk Assessment
• Laboratory Director

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Biosafety Level 1

• Agents not known to cause disease
• Access control
• Minimize aerosols
• Safe handling sharps
• Biohazard signs

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Biosafety Level 2

• Moderate Risk Agents
• Human-derived samples
• Risk Assessment
• Aerosol transmission

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Biosafety Level 3

• Potential for respiratory transmission
• May cause serious or lethal infection
• BSCs
• Access Control
• Negative pressure

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Biosafety Level 4

• High risk fatal disease
• Aerosol route of transmission
• No available vaccine or therapy
• Class III or full body suit with air supply

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Risk Assessment

• Qualitative (unknowns or unlabeled)
• Quantitative
• (residual levels of formaldehyde)
• Laboratory Director responsibility

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Factors for Risk Assessment

• Pathogenicity
• Route of transmission
• Agent Stability
• Infectious dose
• Concentration
• Origin

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Risk Assessment Cont.

• Availability of data
• Availability of effective prophylaxis
• Medical surveillance
• Personnel skill level

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Emergency Procedures

• Develop an emergency plan
• Cuts, accidental punctures
• Ingestion of chemicals/hazardous materials
• Spills
• Broken/leaking containers
• Fire, and other natural disaster
• Facility emergencies
• Power outages
• HVAC failure
• Flood
• Threats
• Emergency Contact Information
• Appropriate and complete medical records should
  be kept
• Write an Incident Report

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Incident Report Form

• Relevant information
• Date and person reporting the incident
• Description of the incident
• Assessment of the problem
• Corrective Action
• Reviewed by the Supervisor/Lab Director

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