Some basic concepts and vocabulary of gene regulation:

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Some basic concepts and vocabulary of gene
regulation 1. Gene action (prok.)
transcription and translation of one or more
specific genes. (euk.) Generally refers to
transcription only. 2. Constitutive genes
gene action at approx. the same level,
regardless of environmental or physiological
changes. Example housekeeping genes. 3.
Inducible genes gene action increases in
response to an increase in concentration of a
substrate, metabolite, etc.). The
latter is called the inducer. 4. Repressible
genes gene action decreases in response to an
increase in concentration of a
substrate, metabolite, etc.). The
latter is called the repressor.
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An operon is a group of closely linked,
functionally related genes, under coordinate
regulation and generally sharing a common
promoter. Usually, operons are transcribed as a
single polycistronic message.
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Isopropyl beta thio-d-galactoside, a gratuitous
inducer.
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Helix-turn-helix or hth regulatory motifs
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The familiar lac repressor protein has HTH motifs
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The zinc finger regulatory protein motif
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The leucine zipper regulatory protein motif
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The receptor protein is the predominant model for
understanding the actions of drugs as well as
steroid hormones
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Ppt preprotachykinin
Alternative splicing of transcripts is an
important form of post- transcriptional
regulation of gene expression
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Note the blue and white colonies on the petri
plate below.
Why are the cells with cloned inserts in the
white colonies and not the blue ones?
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