Supplementary Figure S1

1
Supplementary Figure S1
A
GSTF8LUC
Expression of GSTF8LUC but not CaMV35SLUC
construct is regulated by desensitization GSTF8L
UC (A) or 35SLUC (B) transgenic lines were
pretreated for 18 h with either 1mM auxin, 1mM
H2O2 (HP) or water, prior to treatment with 1mM
H2O2. The bioluminscence of individual seedlings
was measure 6 h after the second treatment and
plotted. The last column contains statistical
analysis using all pair tukey-kramer at 0.05
significance. Non-overlapping circles represent
significant differences between treatment.
B
35SLUC
2
Supplementary Figure S2
2nd Induction 1mM H2O2
Only H2O2 concentrations capable of inducing the
GSTF8 promoter can activate desensitization
Plates containing three rows of four-day
GSTF8LUC seedlings were pretreated with
different concentrations of H2O2 14 h prior to
1mM H2O2. For each treatment, 3mL was added to a
55 mm MS plate containing 50mM luciferin and
decanted after 40 min. The bioluminescence was
measured for each seedling 6 h after after 1st
and 2nd treatment using a CCD camera. The purple
images reflect the fluorescence of the
chlorophyll, while the green represents the
luminescence expression from the active
GSTF8LUC construct.
3
Supplementary Figure S3
Okadaic acid treatment does not induce the GSTF8
promoter Four day old GSTF8LUC seedlings were
treated with 1 mM auxin, 1 mM okadiac aicd (OA)
or water for 40 min and the bioluminescence over
a 19 h time course was measrued using a CCD
camera. The average light units per seedling for
each treatment, with standard errors, are
presented.