Title: Field Micro
1Field Micro
Dee Griffin http//GPVEC.UNL.EDU
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2Supplies
- Sterile syringes and needles
- Ten cc syringes and 20 gage, 1.5 inch needles for
collecting needle aspirates and for inoculating
agar plates. - Butane lighter
- Use a butane lighter for sterilizing aspiration
needle before using needle to streak agar plate. - Culture materials
- Oxidase and Indole reagent droppers BBL
4361181/4361185 (35 per 50 droppers) - Blood, MacConkey, TSI Physician Lab Supply
1014/1006/6032 (3.10 to 3.40 per 10) - Poly O Antiserum
- BBL 40707 (46 per 2 ml, one drop per test)
3Procedure
- Identify target tissues for culture,
- Aspirate - 10cc syringe 1.5 20 g needle
- Spray the aspirate on blood agar plates
- Bend the 1.5 needle in a 45 degree angle
- Flame until sterile
- Use bent needle to streak the agar plate
- Tape the edges of the agar plate.
- Double bag the plate it is ready to ship
4Procedure
- For on site identification you will need the
following identification test and can use the
following chart. - 3 Potassium Hydroxide (Grams)
- Oxidase
- Indole
- Hydrogen Peroxide
- Salmonella Poly O Antiserum
- An incubator can be made from an aquarium heater,
light socket, thermometer, and ice chest. (see
PHAST TEST)
5Incubator
6Making an Incubator
7.
Anaerobic
8Field Streaking
9Bact. ID ?
Too Complicated for a mere mortal
10Microscopes Can Be Useful But not required
11ID
12ID
13Bact. ID
GROUP I Growth on Blood and MacConkey Agar
(GRAM -). Red ? Colony Color
? Colorless E. coli
Salmonella (TSI A/A,
gas,-H2S) (TSIK/A, gas,
/- H2S) (oxidase - )
(oxidase -
) (indole )
(indole - )
Hemophilus s (CO2)
(TSI NC)
(oxidase )
(catalase swine, -
cattle)
(Indole - ) GROUP II Growth on Blood and poor
or delayed growth on MacConkey Agar (GRAM
-) Variable color Pasteurella sp. (TSI
A/Aw) (oxidase ) (catalase ) (indole m, h-
)
14Bact. ID
GROUP III Growth on Blood and no growth (GRAM
) on MacConkey agar. Catalase ()
(-)
. Staphylococcus sp.
Streptococcus sp. (cocci/grapes)
(cocci/chains)
Actinomyces pyogenes
(TSI NC)
(rods/letters)
K alk, A acid, w weak
Grams reaction stringy on 3 KOH Gram (-),
no reaction Gram () Oxidase and Indole Color
test (purple/dark blue) on white tissue paper
(recheck at 48 hrs) Catalase bubbles on 0.3
H2O2 TSI (Triple Sugar Iron) ( Kalk,
Aacid wweak, NCno change) Salmonella Poly O
Antiserum Agglutination ()
http//www.vet.uga.edu/erc/WEBFILES/DEFAULT.HTM
15.
P multocida
16M hemolytica
17E coli
18Salmonella
19Antibiotic Screening - PHAST
- Bacillus megaterium spores
- (ATCC 9885)
- PHAST (Pre-Harvest Antibiotic Screening Test)
- Microbial Inhibition
- Must deal with False Pos. False Neg.
- NOT RELIABLE TO EVALUATE THE RESIDUE STATUS OF AN
ANIMAL WHICH HAS NOT MET THE WITHDRAWAL TIME
SPECIFIED ON AN ANTIBIOTIC LABEL
20Antibiotic Screening
- COLLECTING URINE
- STREAK THE PLATE
- POSITION THE N5 CONTROL DISC
- TEST THE URINE
- INTERPRET THE RESULTS
21Antibiotic Screening
PHAST DOC
22PHAST Results .
23No Loose Parts Necropsy
Dee Griffin http//GPVEC.UNL.EDU
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The Movie
24Steps
25Steps
Necropsy Doc
26Steps
27Collecting the Brain
28Collecting the Brain
29Collecting the Brain
30Collecting the Brain
31Steps
32Sharpening Knives
Dee Griffin http//GPVEC.UNL.EDU
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33It is all about ANGLES
34It is all about ANGLES
35Motorized ANGLES
36Testing the Edge
37Using a Steel
38Syringe Care
Dee Griffin http//GPVEC.UNL.EDU
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39Microwave beyond popcorn
40Mic the disposable autos
41Mic the disposable autos
42How To Give Dehorning Anesthetic
43Why Give An Anesthetic
- Stops most, if not all of the fight
- Decreases most, if not all of the bawling
- Make the job a bit easier frequently safer
- Potential to improve performance
- We sell performance gain efficiency
- If an animal we depend on to make a living is
mistreated in any fashion physically,
nutritionally, environmentally, emotionally any
fashion it comes out of our bank account! - It is a nice thing to do
44What Do You Need
- Lidocaine see your vet
- just like most of the new antibiotics you are
using, it is a prescription item - 18 gage needle 5/8 to 1
- Syringe that will deliver 5 to 10 cc
lidocaine
45How Does It Work
- Lidocaine infused in or around the CORNEAL nerve.
- Pain control is almost immediate if given in or
next to the nerve.
46How To Give It
- Palpate the CREST or RIDGE between the eye
the horn. - Slide needle ½ to ¾ straight in under the ridge
or crest. - Inject 5 to 10 CC lidocaine.
47Inject half way between eye horn ½ - ¾
deep under the ridge
Anatomy
Using Plastic syringe
Using Automatic syringe
Palpate Ridge
MOVIE
48DGRIFFIN_at_GPVEC.UNL.EDU http//GPVEC.UNL.EDU
Save a Cow Eat a Vegetarian
Good Luck To You
49Biosecurity in the real world? can you say
A-RITS
50Best Way To Deal With Disease
51(No Transcript)
52Health - Disease Complex Interactions
53Biosecurity Major Components
- Assess
- Resist
- Isolate
- Traffic
- Sanitation
-
- RITS are a set of hurdles
54Biosecurity Risk Evaluate
- Accessibility
- Personnel training
- Reservoir(s)
- Isolation
- Traffic Flow
- Sanitation
- Pest Control
55Biosecurity Risk Evaluate
- Accessibility
- Personnel training
- Reservoir(s)
- Isolation
- Traffic Flow
- Sanitation
- Pest Control
56Biosecurity Specific Disease Control
Identification
- Risk
- Training
- Isolation
- Traffic Control
- Sanitation
- Action Trigger
- Rapid Response
- Comments
EXAMPLES BS-Man-07-SpecficDisContID.doc
57Risk Management - PRCE
- Prevent, Reduce, Control, Eliminate
- Resistance General ? Specific
- SOURCE, EXPOSURE Additions
- Isolate, test, monitor, re-test
- Traffic Control
- Sanitation
- Justify ? Verify ? Monitor
- Is there evidence for control?
- Is it getting done?
- Is it working? (surveillance testing)
58Secret
59Sanitation Disinfectants
- Sanitation should stress
- CLEAN not the use of disinfectants
- Selection and use of Disinfectants
- Understand the target pathogen
- Understand the organic load
- Understand the disinfectant properties
- Understand disinfectant have been documented to
relate to antibiotic resistance
60Disinfectant Classification
- Hypochlorites
- Iodine and ionophore disinfectants
- Chlorhexidine
- Alcohols
- Oxidizing Agents
- Phenolic disinfectants
- Quaternary Ammonium Compounds
- Aldehydes
61Disinfectant Properties .
All About Disinfectants
62Disinfectant Virus Targets
Go to End
63Biosecurity-Security Emergency Action Plan
- Assessing the situation
- Is it a disease concern?
- Is it a contaminate concern?
- Is it an intruder concern?
- NOTIFY THE PROPER PERSONNEL
- IMPLEMENT QUICK RESPONSE PLAN
- SANITATION SECURITY
Go to End
64(No Transcript)