EUKARYOTIC TRANSCRIPTION

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EUKARYOTIC TRANSCRIPTION
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CHAIN INITIATION
CHAIN ELONGATION
CHAIN TERMINATION
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What is another difference between
prokaryotic and eukaryotic chromosomes?
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5' CAP
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1) What do you think will happen if the RNA
moelcule isn't capped? 2) Contrast that
with Prokaryotes.
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CHAIN INITIATION
CHAIN ELONGATION
CHAIN TERMINATION
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CHAIN INITIATION
CHAIN ELONGATION
CHAIN TERMINATION
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Is the poly-A tail encoded in the genome?
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primary transcipt pre-mRNA
mRNA
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Intron Splicing
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What the hell is 5, 3 or 2. What are those 5
to 3 linkages, 5 to 5 linkage, or 2 to 5
linkage?
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If RNA utilized dNTP instead of NTP, could you do
the splicing reaction?
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You have to bring the branch point and the 5'
splice site close to each other for the first
cleavage to occur, and then you have to bring the
5' splice site and the 3' splice site together
for the second reaction (attachment) to
occur. If you had to design a spliceosome to do
that, what kind of molecule would it have and
what would be its properties?
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pre-mRNA GAUGUCGAGUGAGGGAGUGAAGCUGCAG
Splice out this intron!
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Sequence of DNA is sequence of primary transcript
primary transcipt (hnRNA)
mRNA
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Does the cell need to have mechanisms to
fix/handle errors in transcription or in
splicing?
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Nuclear Export of mRNA
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What about other RNAs?
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