Nanobioprocessing

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Nanobioprocessing

• Larry P. Walker, Ph.D.
• Professor, Department of Biological and
  Environmental Engineering
• Cornell University

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Agricultural and Environmental Bioprocess
Engineering Research

• Molecular mechanisms of polysaccharide degrading
  enzymes,
• Solid state microbial processes and molecular
  ecology,
• Mechanisms and kinetics of metal uptake and
  sequestration in plants,
• Molecular filtration and analysis of biomolecules

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Nanobiotechnology Center (NBTC) Cornell University
The Nanobiotechnology Center (NBTC) was
established in January 2000 as a Science
Technology Center, with core funding from the
National Science Foundation. Nanobiotechnology is
an emerging area of scientific and technological
opportunity that integrates nano/microfabrication
and biosystems to the benefit of both.
www.nbtc.cornell.edu
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Nanobiotechnology Center (NBTC) Cornell University
Nanobiotechnology is beginning to generate
substantial new insights into how biological
systems function, and likewise, nanobiotechnology
will lead to the design of entirely new classes
of micro- and nanofabricated devices and systems.
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Nanobiotechnology Center (NBTC)Cornell
University

• Cornell University
• Clark Atlanta University
• Howard University
• Princeton University
• Oregon Health Sciences University
• Wadsworth Center New York Department of Public
  Health

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Nanobiotechnology Center (NBTC)Cornell
University

• Cellular Devices (David Lawrence, Wadsworth
  Center) studies of engineered devices for
  separating and analyzing individual cells.
• Nanoscale Cell Biology (Manfred Lindau, Cornell
  University) - micro- and nanofabricated tools to
  understand and utilize cellular responses on the
  nanoscale.
• Biomolecular Dynamics (Michael Koonce, Wadsworth
  Center) exploration and utilization of
  molecular motion and mechanical properties

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Nanobiotechnology Center (NBTC)Cornell
University

• Cell-Surface Interactions (Harvey Hoch, Cornell
  University) Investigating the response and
  control of cellular responses on structured
  surfaces
• Biomolecular Devices and Analysis (Larry Walker,
  Cornell University) - investigation new
  approaches to high speed and highly sensitivity
  analysis of biomolecules.
• Nanoscale Biomaterials (Dotse Sogah, Cornell
  University) study of materials engineered at
  the nanoscale for new properties that can be
  exploited in nanobiotechnolgy.

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Biomolecular Devices and Analysis
Major research and development activities in the
life sciences has generated the need for
materials, methods, and devices for sorting,
separating, and analyzing proteins, DNA and other
biomolecules.
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Membranes and Packings
Semipermeable Membranes and Equilibrium
Dialysis Michael Spencer, Lori Lepak, Jim Turner,
Michele Caggana
Fabrication of Integratable Semipermeable
Membranes Jim Turner, Peter Russo, David Martin,
David Lawrence, Michele Caggana, Bill Shain,
Michael Isaacson, and Michael Spencer
Porous Block Copolymers For Microfluidic
Separation Chris Ober Mingqi Li
High Resolution Protein Purification using Block
Copolymer Derived Mesoporous Silica-type
Materials Uli Wiesner, Phong Du, Caroline Corner,
Larry Walker
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Micro and Nanofluidic devices
Micro and Nanofluidics for Molecular Separation
and Detection Harold Craighead, John Henion,
David Czaplewski, Yanou Yang Jun Kameoka
Multidimensional Microscale Separations of
Cerebrospinal Fluid Proteins Kelvin Lee, Michael
Shuler, Jim Engstrom, Bob Austin, Chen Li
Jinpian Diao
Nanostructures for Enzyme Transport and
Accessibility Assessment Larry Walker, Harold
Craighead, Jennifer Guisado Tina Jeoh
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Biomolecular Devices and Analysis
Some Common Activities

• Lithography
• Chemical synthesis
• Spinning of molecules
• Assessing morphology
• Assessing monodispersity
• Assessing transport mechanisms
• ? Measurement Analysis

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Biomolecular Devices and Analysis

• Biocompatibility,
• Working with small volumes pico- to
  micro-liters,
• Sieving and sorting at the scale of 5, 10, 15 and
  50 nm,
• Monodispersity tight control over pore size
  distribution and shape

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Example Membrane Technology
Spin Coating of Cellulose And Collagen onto
silicon surfaces to create porous membranes
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Fabrication of Integratable Semipermeable
Membranes M. Spencer
400 nm thick
Collagen
Pores 0-100nm
Dyes of 270 390 Da
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Block Co-polymersUli Wiesner Chris Ober

• ? excellent biocompatibility of silica
• easily accessible pore sizes from 8-50 nm
• narrow pore size distributions

Chemical Synthesis
Photo Lithography
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Nanostructures for Enzyme Transport and
Accessibility

• Studying cellulase binding and catalysis as a
  function of molecular diameter.
• Study synergism in cellulase/ cellulose reaction
  systems.

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Multidimensional Microscale Separations Proteins
K. Lee
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Micro and Nanofluidics for Molecular Separation
and Detection H. Craighead
Electrospray Ionization
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Application to Mass Spectrometry Analysis
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NBTC At the Interface between Biology and
Engineering
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We are having fun!