Oxidative Burst

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Oxidative Burst

• Doug Tischer

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General Idea
Galactose oxidase
State 1
No signal
Catalase
Buffers the cell from any leaky GOase expression
State 2
Galactose oxidase
Signal
Catalase
(Bad cell)
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System Design
luxR
GOase
tetR
LuxR
katEssrA
pTET
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System Response

• KatE degradation lags behind GOase production

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Parts Breakdown
luxR
GOase
tetR
lux pR
lacI pL
B0034
F2622
Q04400
From Arnold lab
B0015
B0034
Q04400
BBa_M0050
Genomic PCR
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Status
7
Connection to other projects

• Source of galactose could be from lactose
  breakdown.
• Cells could be triggered to adopt this fate from
  SSM.
• ROS species could be detrimental to vitamin
  synthesis (among many other things).
• Vitamins could be harmful to ROS generation if
  they are antioxidants.