Sequence Analysis using Bioinformatics tools

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Sequence Analysis using Bioinformatics tools

• Group 2
• Francisco Cubillos
• Amin Jalili

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Sequence

• 240 bp sequence
• Organism?

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Blast
Organism A. thaliana Gen gai
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Gene Information

• Sequence belongs to gene gai
• Chromosome 1
• Locus AT1G14920

Exons 1 Transcript length 2,129 bps
Translation length 533 residues
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Blast
TIGR
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Comparison in ortologus sequencesCLUSTAL W
Arabidopsis thaliana Brassica campestris Vitis
Vinifera Gossypium barbadense Lycopersicon
esculentum
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GRAS Motif

• Pfam tool
• GAI, RGA, SCR
• Proteins in the GRAS family are transcription
  factors that seem to be involved in development
  and other processes

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Transcription Factor

• GAI is similar to a putative transcription factor
  and transcriptional coactivators.
• Repressor of GA responses and involved in
  gibberellic acid mediated signaling. Member of
  the DELLA proteins that restrain the cell
  proliferation and expansion that drives plant
  growth.

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Expression profile in leafs

• No differences were observed in the different
  leafs
• Measure expression profiles in other tissues to
  detect specific responses

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GAI Protein

• Translation length 533 residues

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Localization
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Interactions
GA1 gibberellin biosynthesis, chloroplast
GID2 GA signaling, chloroplast SPY GA
signaling, nucleus
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Mutant phenotype
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Conclutions

• Transcription factor involve in the gibberellic
  acid pathway.
• Activates response of the cell in the presence of
  GA