AntiBacterial Properties of Silver Nanoparticles

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Anti-Bacterial Properties of Silver Nanoparticles
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Objective

• You will learn about
• Nanotechnology
• Making nanoparticles
• Applications of nanoparticles

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Motivation

• Technology involving features with a size between
  1 and 1000 nanometers can be considered
  nanotechnology.

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Motivation

• Technology involving features with a size between
  1 and 1000 nanometers can be considered
  nanotechnology.

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Motivation

• Nanotechnology is an emerging industry which is
  bringing us exciting new products and promises to
  change the way we live and work in the future.

Nanowire Chemical Sensor
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Motivation

• Nanotechnology is an emerging industry which is
  bringing us exciting new products and promises to
  change the way we live and work in the future.

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Food containers

• Your food stays fresher longer so you throw away
  less. (How much cash do you throw in the trash?)

http//www.bluemoongoods.com/silver_nanoparticle_f
ood_containers.htm
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Baby bottles

• Through silver nano poly system, 99.9 of germs
  are prevented and it maintains anti-bacterial and
  deodorizing functions as well as freshness

http//babydream.en.ec21.com
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Toothpaste

• Prevent gum inflammation, periodontitis, and
  periodontal disease
• Sterilizes and disinfects the Bacilli that is a
  major cause for all gum disease by pure silver
  powder of purity 99.99

http//www.gnsnanogist.com/
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Toothbrush

• Songsing nanotechnology company

http//www.ssnano.net/ehtml/detail1.php?productid
73
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Cuttingboard

• Antibacterial Effect by Nano Silver Clean

http//www.adox.info/?docshop/item.phpit_id0001
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Computer mouse

• IOGEAR's Personal Security Mouse is coated with a
  Titanium Dioxide (TiO2) and Silver (Ag)
  nano-particle compound. The coating uses two
  mechanisms to deactivate enzymes and proteins to
  prevent a wide spectrum of bacteria, fungi, and
  algae from surviving on the surface of the
  mouse.

www.iogear.com/main.php?locproductItemGMEFP1W6
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Antibacterial athletic sock

• Cushioned, fitted, quarter-length sports socks
  knitted with a cotton material containing
  millions of invisible silver nanoparticles.
• Innovative socks are naturally antibacterial
  and antifungal

http//www.sharperimagebest.com/zn021.html
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Cotton sheets

• Whether you are looking for health benefits, the
  feeling of extra freshness or just the feeling of
  100 cotton sheets, AgActive sheet sets are the
  definition of luxury and can also help in the
  fight against cross infection of super bugs such
  as MRSA.

http//www.agactive.co.uk/index.cfm/fuseaction/pro
duct.display/Product_ID/8/.htm
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Washing machine

• Globally recognized for its high-tech and
  futuristic appliances, Samsung incorporates a
  technology called Silver Nano. C1235A 5.2 kg Drum
  Type washing machine comes with Silver Wash that
  sterilizes your clothes. Plus with its stainless
  steel tub, it can help you save water up to 30

http//www.samsung.com/ph/products/washingmachine/
drum/c1235a.asp?pageSpecifications
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Today's activities

• Make nanoparticles
• Soak filter in nanoparticles
• Inoculate plate
• Check results

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How to make silver nanoparticles

• Put 2 ml .5 mM AgNO3 in a test tube
• Start with a compound that has silver in it
• Our solution is silver nitrate

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How to make silver nanoparticles

• Put 2 ml .5 mM AgNO3 in a test tube
• Heat in boiling water bath for 5 minutes
• Heating it will speed the reaction

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How to make silver nanoparticles

• Put 2 ml .5 mM AgNO3 in a test tube
• Heat in boiling water bath for 5 minutes
• Add 5 drops of 1 sodium citrate
• Carefully add the sodium citrate,
• the solution is HOT
• Allows gold to form stable nanoparticles

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How to make silver nanoparticles

• Put 2 ml .5 mM AgNO3 in a test tube
• Heat in boiling water bath for 5 minutes
• Add 5 drops of 1 sodium citrate
• Continue heating wait for silver nanoparticles
  to form.
• Watch for a change in color to indicate the
  silver has formed
• Let it heat a few more minutes to be sure the
  color change is complete

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How to make silver nanoparticles

• Put 2 ml .5 mM AgNO3 in a test tube
• Heat in boiling water bath for 5 minutes
• Add 5 drops of 1 sodium citrate
• Continue heating wait for silver nanoparticles
  to form

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Growth of bacteria

• Bacteria may grow as a group colony
• Bacteria may cover surface of plate lawn

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Bacterial antibiotic sensitivity

• Antibiotics may inhibit the growth of some
  bacteria
• Evidence of this is a halo
• A halo indicates a zone where bacteria are not
  present

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Procedure

• Cut filter paper into small squares
• Place squares in a petri dish and pour silver
  nanoparticles over them
• Let the squares soak for about 10 minutes

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Procedure

• Mark the bottom of an agar plate with your
  initials, divide the bottom into sections, and
  label
• Put 1 to 2 drops of bacterial culture on the
  plate
• Spread the drops

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Procedure

• Place your nanoparticle soaked filter paper
  squares and your control(s) in the designated
  areas
• Incubate your plate for 24 hours at 37ºC

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Questions
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END

• The following slides display results and are
  included only as a teacher resource for a
  discussion after the experiment has been
  completed.

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Results
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Results
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Results
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Results
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Results
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Results
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Results