SynBio

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SynBio iGEM StoriesMon 8-May-2006
Your role(s) Parts Devices Systems Selections
Technologies Tests datasheets
40 teams 10 people 2M / quarter yr
http//parts.mit.edu/r/parts/partsdb/view.cgi?part
_id5114
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The 2004 iGEM poster was synthetic, but not
biological
( an artifact of light sensitive dyes)
Privately rehabilitated in time for 2005 iGEM
jamboree then publication Levskaya A, et al.
Nature 2005 Nov 24 Synthetic biology
engineering E.coli to see light.
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iGEM Goals Past Future
A synthetic multicellular system for programmed
pattern formation Basu, et al. Nature 4341130
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iGEM Goals Past Future
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Synthetic combinatorics selection for secretion
Second Passage
First Passage
?trp/?tyrA pair of genomes shows the best
co-growth Reppas, Lin Church Shendure et
al. Accurate Multiplex Polony Sequencing of an
Evolved Bacterial Genome(2005) Science 3091728
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Environmentally controlled invasion of cancer
cells by engineered bacteria. Anderson et al. J
Mol Biol. 2006
Optical imaging bacteria, viruses, and mammalian
cells encoding light- emitting proteins reveal
the locations of primary tumors metastases
in animals. Yu, et al. Anal. Bioanal. Chem.
2003. accumulate in tumors at ratios in excess
of 10001 compared with normal tissues.
http//www.vionpharm.com/tapet_virulence.html
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LPS- Capsule Dap- for safety
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Engineering a mevalonate pathway in Escherichia
coli for production of terpenoids. Martin VJ, et
al. Nat. Biotech 2003
Production of the antimalarial drug precursor
artemisinic acid in engineered yeast. Ro DK, et
al. Nature. 2006 8
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Programmable ligand-controlled riboregulators of
eukaryotic gene expression.
OFF
ON
ON
Bayer Smolke 2005 Nature Biotech.
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Combinatorial characterization
P1-GFP-RegP2-RiboRegCR-Output-AA-Stop-Term-P3-Ribo
RegTA. 50 900 50 25 900 30 3 25
50 90 bp 1 1 10 10 6 10
1 1 10 1E3 combinations ( 6E8 total)
This gives analog logic (P2, P3 riboreg)
which can be characterized in situ to
quasi-automatically produce pages for a
parts properties handbook. RegP2,P3 lac, tet,
ara, heat, cold, cell-cycle, light(2), quorum,
hypoxia(3) RiboRegCR cis-regulator
non-cross-reacting 5 types two
strengths. RiboRegTA Smolke, Isaacs -- Explore
variations on the tiny ligand-specifying region.
Output Ds-Red, cat, kan, luciferase, tetR,
RiboReg2, biosynthetic enzyme AA nil, lite,
Flag, his-tag, PKS-association,
leu-zipper BBa_R0053 BBa_B0030 BBa_E0040
BBa_B0011 BBa_I0500 BBa_J01010
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Combinatorial Synthesis ofGenetic Networks
Guet et al. Science 2002
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Combinatorial Synthesis ofGenetic Networks
Guet et al. Science 2002
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Nutriceuticals cosmetics
The FDA does not require disclosure of color or
flavor additives if GRAS ("generally recognized
as safe"). A natural flavor is not necessarily
more healthful or purer than an artificial one.
When almond flavor -- benzaldehyde -- is derived
from natural sources, such as peach and apricot
pits, it contains traces of hydrogen cyanide, a
deadly poison. Benzaldehyde derived by mixing oil
of clove and amyl acetate does not contain any
cyanide. Nevertheless, it is legally considered
an artificial flavor and sells at a much lower
price. Feb 2005 the FDA issued an unprecedented
warning to the cosmetics industry stating that
the Agency is serious about enforcing the law
requiring companies to inform consumers that
personal care products have not been safety
tested. http//www.rense.com/general7/whyy.htm
http//www.ewg.org/issues/cosmetics/FDA_Warning/
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NIH Guidelines Recombinant DNA
molecules that are constructed outside living
cells by joining natural or synthetic DNA
segments to DNA molecules that can replicate in a
living cell.    U.S. National Organic Standards
Board definition of genetic engineering GMOs
"Made with techniques that alter the molecular or
cell biology of an organism by means that are not
possible under natural conditions or processes
It shall not include traditional breeding,
conjugation, fermentation, hybridization,
in-vitro fertilization, or tissue
culture." http//www4.od.nih.gov/oba/rac/guidelin
es_02/NIH_Guidelines_Apr_02.htm_Toc7261549
http//www.omri.org/OMRI_GMO_policy.html
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Conjugation
Rapid alternative to DNA transformation?
Conjugation between bacterial and mammalian
cells. Waters VL. Nat Genet. 2001
Transformation of isolated mammalian
mitochondria by bacterial conjugation. Yoon YG,
Koob MD. Nucleic Acids Res. 2005
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