Diapositive 1

1
MOLECULAR CARACTERISATION OF SOFT TISSUE SARCOMAS
WITH COMPLEX GENETICS
Frédéric Chibon Pauline Lagarde - Jean-Michel
Coindre - Alain Aurias Tumor Genetics Département
of Pathology Insititut Bergonie Bordeaux -
FRANCE
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GENETIC CLASSIFICATION OF STS
Sarcomas with recurrent translocations
Sarcomas with oncogenic mutations
Sarcomas with simple genetics
Sarcomas with a complex genetic profile
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SARCOMAS WITH A COMPLEX GENETIC PROFILE
Leiomyosarcomas Adult Rhabdomyosarcomas Pleomorp
hic Liposarcomas Myxofibrosarcomas Poorly-differ
entiated sarcomas / MFH
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SARCOMAS WITH A COMPLEX GENETIC PROFILE
Different prognosis but. Difficult to
classify ! share similar morphology Share
histological patterns with dedifferentiated
liposarcomas Genetic mechanisms still poorly
understood
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French Sarcoma Group Database
2538 adult STS with untreated primary
tumor (January 2006)
Well differentiated and dedifferentiated LPS
262 (10.3) LMS, adult RMS, pleomorphic LPS,
MFS and Poorly-Differentiated Sarcomas (PDS) /
MFH 1183 (46.6)
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COMPLEX GENETIC PROFIL OF SOFT TISSUE SARCOMAS
Analyzed tumors CGH ARRAY 203 cDNA ARRAY 170
LPS DD 30
MFH-PDS 35
LMS 35
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Two main subgroups of genetic profiles
DD LPS
LMS
MFH PDS
Amplification
Gain
Loss
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DD LPS Simple Genetics based on co-amplifications
Amplification
Gain
Loss
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Two different profils correspond to two different
types of LMS
LIMB
TRUNK
Ext
Int
Amplification
Gain
Loss
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Identification of driver genes in amplifications
CGH ARRAY
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Genetic alterations and clinical datas
5p Amplification One gene amplified,
up-regulated.... ...Wich effect on the tumor
biology ?
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CONCLUSION
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MOLECULAR CARACTERISATION OF STS WITH COMPLEX
GENETICS
Jean-Michel Coindre Binh Nguyen Bui Pauline
Lagarde Véronique Brouste Cécile Garcia
Alain Aurias Caroline Louis
11 CLCC French Sarcoma Group Pathologist