Activity

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• Activity 2 Ethanol and Tobacco Toxicity in
  Living Animal Cells

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Step 1

• Infusion of hay, dry grass, or pond mud
• Opacity Check try reading a newspaper through
  the culture jar to check for cloudiness

Infusion
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Step 2 - Treatments

• Add various substances to other culture tubes
• Water (leaves one tube untreated as a control)
• Alcohol, tobacco
• Here we also tested mouthwash and toothpaste

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Step 3a Examine Tubes Visually
Water (control)
Mouthwash
Toothpaste
Coca Cola
Tobacco
Ethanol
Observe and Note Immediate Effects (Repeat
again after two days)
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Step 3b Inspect the Organisms
Examine Organisms Under the Microscope
Initially and Two Days Later
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(No Transcript)
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Step 4. Effects of Treatment Over Time
As seen under a microscope, number and type of
protists should change rapidly in a matter of
hours
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Step 5
DNA Extraction
Water (control)
Mouthwash
Coca Cola
Toothpaste
Tobacco
Ethanol
DNA
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Water (control)
DNA
Mainly Salt
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Ethanol
Mainly Salt
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Tobacco
DNA
Salt
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Mouthwash
DNA
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Toothpaste
DNA
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Coca Cola
DNA