Protein Synthesis

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Protein Synthesis
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One Gene One Enzyme
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Protein Synthesis
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Protein Synthesis
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Protein Synthesis
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Protein Synthesis
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Protein Synthesis
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Protein Synthesis

• Transcription
• DNA ? mRNA
• Occurs in the nucleus

• Translation
• mRNA ? tRNA ? AA
• Occurs at the ribosome

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Triplets (Codons)
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Genetic Code
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Transcription
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Transcription
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Transcription
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Transcription
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Initiation

• Promoter
• TATA Box
• Initiation Site
• Transcription factor
• RNA Polymerase
• AUG

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Elongation

• RNA Polymerase
• Adds nucleotides to the 3 end

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Termination

• Proceeds until RNA polymerase transcribes a
  terminator sequence
• UAA
• UAG
• UGA

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RNA Processing

• 5 guanine cap
• 3 Poly (A) tail

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RNA Splicing

• Introns
• Exons

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Spliceosomes

• snRNPs
• Small nuclear ribonucleoproteins

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Introns????

• Regulation
• One gene can code for alternate polypeptides
• Evolution
• Domains structural and functional regions

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Translation
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tRNA
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Aminoacyl-tRNA Synthetase
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Ribosomes

• Small subunit
• Large subunit
• P site (peptidyl-tRNA)
• A site (Aminoacyl-tRNA)
• E site (Exit site)

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Initiation
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Elongation
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Termination
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Polyribosomes
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Point Mutation
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Point Mutation

• Base Pair Insertion or Deletion
• Frameshift
• Missense new AA
• Nonsense stop

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Point Mutation

• Base Pair Substitution

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(No Transcript)