BIOSCAN S'A'

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BIOSCAN S.A.

• Chile - 2006

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Summary

• Mission/Vision
• Company history
• Laboratory
• Detection of human pathogens
• Detection of plant viruses
• Research

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Interest Areas

• Molecular diagnostic and clinical trials
• Seaweeds small molecules with biological
  activities

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Chart
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Directory

• Chairman Rinaldo Zecchetto, Industrial Civil
  Engineer.
• Director Alejandra Moenne, PhD, Biochemistry and
  Molecular Biology.
• Director Pier Zecchetto, Industrial Civil
  Engineer.
• Director Patricio Arce, PhD, Plant Molecular
  Biology.
• CEO Roberto Valladares, Industrial Civil
  Engineer.

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Scientific Advisory Committee

• Scientific Director Patricio Arce, PhD, Plant
  Molecular Biology. Pontificia Universidad
  Católica de Chile.
• Scientific Advisor Alejandra Moenne, PhD,
  Biochemistry and Molecular Biology. Universidad
  de Santiago de Chile.
• Medical Director MD. Antonio Banfi, Pediatrics.
  Universidad de Chile.

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History

• 1994. Began operation by developing the PCR
  detection of 12 periodontal pathogens
• 1996. Development of gynecological pathogens
  detection
• 1998. First international publication. Am. J.
  Obst. Gynecol. 1998 179 1115-9.
• New stockholders June 2005

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History

• 2004. PCR detection of 23 RNA viruses in fruit
  trees. (CORFO grant)
• 2005. New facilities
• 2005. Patent pending algal sulfated galactan, as
  a plant immunity stimulator
• 2005. Fruit fingerprinting project (FONDEF grant)

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Facilities and Personnel
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Facilities and Personnel

• 330 m2
• Molecular biology infrastructure including 4
  separated working areas
• Unit of biosafety level II
• 14 staff

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Human Pathogens Detection
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Human Pathogens Detection

• PCR detection of 50 human pathogens
• 160.000 detections
• CAP Surveys Program ID and HC-6
• 25 hospital an clinics
• 400 physicians
• LCR, LA, uterine, tissue, blood and sputum
  specimens

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Commercial Activities

• Medical visit and presentation to clinicians of
  different specialities gyneco-logy and
  obstetrics, dermatology, urology, pediatric,
  critical care, pulmonary, etc.
• Contact with hospitals ethic committees and
  regulators
• Congress 3 people sale team
• Presentation in Congresses
• Commercial agreements with hospitals and clinics
  laboratories

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Human Health Customer

• Clínica Las Condes
• Clínica Tabancura
• Clínica Indisa
• Hospital Luis Calvo Mackenna
• Hospital Luis Tisné
• Hospital San Borja
• Other Clinic and Hospital(19)
• Medical Center (45)
• Medical offices (330)

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Gynecological Pathogens

• Human papilloma virus
• HPV types 6, 11, 16, 18, 31, 33, 45, 56, 58, 59
• Streptococcus agalactiae
• Chlamydia trachomatis
• U urealyticum/parvum
• Mycoplasma hominis
• Neisseria gonorrhoeae
• Treponema pallidum
• Candida spp.
• Trichomonas vaginalis
• Enterococcus spp.
• Escherichia coli
• Proteus spp.

• Herpes simplex virus 1
• Herpes simplex virus 2
• Gardnerella vaginalis
• Mobiluncus spp.
• Prevotella spp.
• Porphyromonas spp.
• Bacteroides fragilis
• Peptostreptococcus anaerobius
• Fusobacterium spp.
• Staphylococcus aureus
• Actinomyces israelii
• Listeria monocytogenes
• Haemophilus influenzae

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Periodontal Pathogens

• Streptococcus mutans
• Actinobacillus actinomycetemcomitans
• Porphyromonas gingivalis
• Prevotella intermedia
• Tannerella forsythia
• Treponema denticola
• Fusobacterium nucleatum
• Peptostreptococcus micros
• Campylobacter rectus
• Eikenella corrodens
• Prevotella nigrescens
• Capnocytophaga spp.
• Streptococcus intermedius

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Investigations

• Amniotic Fluid Pathogens detection
• Hospital Clínico Universidad Católica
• Streptococcus agalactiae, Mycoplasma and
  Ureaplasma detection in pregnant women
• Hospital Luis Tisné
• Clínica Las Condes
• Treponema pallidum detection in newborn children
• Hospital Padre Hurtado

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Investigations

• Gynecological Pathogens PCR detection
  (Ureaplasma, Mycoplasma, HPV, G. vaginalis,
  Prevotella spp., Candida spp. y N. gonorrhoeae)
• Universidad de los Andes/CUEM Hospital
  Parroquial San Bernardo
• Effect of C. trachomatis infection in tubal
  pregnancies
• Hospital Parroquial San Bernardo

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External Quality Control
CAP (College of American Pathologists) Surveys
Program

• HC6 Chlamydia/NGC Nucleic Acids Amplification
• Chlamydia trachomatis y Neisseria gonorrhoeae
  PCR detection
• ID Human Papillomavirus y Human herpesvirus 1-2
• HPV and HSV 1-2 PCR detection

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Development

• Herpes virus family Zoster (type 3),
  Epstein-Barr (type 4), Cytomegalovirus (type 5)
• Mycobacterium tuberculosis complex
• (M. tuberculosis, M. microti, M. bovis, M.
  africanum, M. canetti)
• Mycobacterium avium complex
• (M. avium, M. intracellulare)
• Bordetella pertussis, Mycoplasma pneumoniae,
  Chlamydia pneumoniae, Legionella pneumophila

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Plant Viruses Detection
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Project Description

• Detection of 23 viruses in grapes, pipfruit and
  stonefruits by RT-PCR
• Project Importance
• - There is no plant antiviral
• - Sanitary authorities check viruses to
  consider a plant as virus-free
• - Viruses decrease production and quality of
  fruit and wines
• Started January 2005

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Team

• Head Investigator Alejandra Moenne, Biochemist,
  PhD Molecular Biology
• Project Manager Carolina Uquillas, Agronomist,
  PhD Molecular Biology
• Investigators Luis Lizama, Biochemist and
  Jeannette Vera, Biochemist.
• Bioscans laboratory team

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Grapevine Viruses

• Arabis mosaic
• Grapevine fanleaf virus
• Grapevine fleck virus
• Grapevine leafroll associated virus 1 to 7
• Grapevine rootstock stem virus
• Grapevine vitivirus A
• Grapevine vitivirus B
• Rupestris stem pitting-associated virus

• Strawberry latent ringspot virus
• Tobacco ringspot virus
• Tomato ringspot virus

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Pipfruits and Stonefruits Viruses

• PIPFRUITS
• Apple chlorotic leafspot virus
• Apple mosaic virus

• STONEFRUITS
• Apple chlorotic leafspot virus
• Cherry leafroll virus
• Plum pox virus
• Prune dwarf virus
• Prunus necrotic ringspot virus
• Tomato ringspot virus

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Sales and Marketing

• First sale in September 2005, grapes virus.
• Contacts established with 10 top nurseries y 5
  main vineyards

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Research and Development
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Development Projects

• 40 kits for real time PCR for human pathogens
  detection respiratory, gineco-obstetric,
  perinatal and pediatric (A.Banfi L.Lizama)
• Bioprospection of algal small molecules with
  biological activities (A.Moenne J.Correa)
• Grape vine, apple, pear, peach and strawberry
  fringerprinting (P.Hinrichsen, INIA)

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Research Projects

• Sulfated Galactan potential plant antiviral
  (A.Moenne)
• Virus cleaning for grapes, pipfruits and
  stonefruits master trees