BIOLOGY

1
BIOLOGY of COMPLEMENT
2
COMPLEMENT
BIOLOGY
DIAGNOSIS
3

C4d
4
C3
injury

C4d
5

C3
injury
C4d
MBL
CRP
Apoptotic
CMV cells
6

C3
injury
C4d
MBL
Regulators
CRP
Apoptotic
CMV cells
7
protection

C3
injury
C4d
MBL
Regulators
CRP
Apoptotic
CMV cells
8
Three Pathways of Complement Activation
Classical
C1
C4
Lectin
C2
C5
C3
MBL
B
Alternative
9
Three Pathways of Complement Activation
Classical
C1
C4
b
a
a
Lectin
C2
C5
C3
b
b
a
MBL
a
B
b
Alternative
10
C4bC2a
C3bBb
C3
C4
C1
C2
C5
C6
C7
C8
C9
0.6 mg/ml
1.3 mg/ml
11
C4bp
DAF CR1
Factor I
MCP
Factor H
DAF CR1
Factor I
C4bp CR1
Factor I
Vitronectin
MCP
Clusterin
C4bC2a
C1inh
CD59
C3bBb
C3
C4
C1
C2
C5
C6
C7
C8
C9
0.6 mg/ml
1.3 mg/ml
12
(No Transcript)
13
(No Transcript)
14
(No Transcript)
15
FUNCTION
C3b gt C4b CR1
iC3b gt iC4b CR3
C3d gt C4d CR2
16
C4d vs C3d STAINING
17
C4d Deposition ATGAM vs Thymoglobulin
C4d / Total
ATGAM
9 / 9
(Horse polyclonal)
Thymoglobulin
0 / 8
(Rabbit polyclonal)
18
(No Transcript)
19
C Reactive Protein Activation of Complement
20
(No Transcript)
21
ANTIBODY ASSOCIATED REJECTION
7 years after transplantation anti-DR7 and DRw53
22
ANTIBODY ASSOCIATED REJECTION
after plasmapheresis plus IVIg anti-DR7 and
DRw53 undetectable
C4d
C3d
23
Clearance of iC3b and C3d
from Human Endothelial Cells in vitro
24
LEUKOCYTE ACTIVATION BY
SUBLYTIC MAC
25
ACTIVATION OF ENDOTHELIAL CELL BY MAC
MAC
W-P
Endothelial
26
ACTIVATION OF ENDOTHELIAL CELL BY MAC
MAC
vWf
CD63
P-selectin
Endothelial
Retraction
Tissue
Factor
27
ACTIVATION OF ENDOTHELIAL CELL BY MAC
MAC
PAF
vWf
CD63
P-selectin
MCP-1
Synthesis
Tissue
IL-8
Factor
Endothelial
Retraction
P-selectin
Tissue
Factor
bFGF
PDGF
28
Monocyte/
P-selectin
bFGF
Macrophage
MAC
PDGF
Patelet
vWf
Tissue
TNFa
vWf
CD63
PAF
Factor
P-selectin
MCP-1
Synthesis
Tissue
IL-8
Factor
Endothelial
Retraction
P-selectin
Tissue
Factor
bFGF
PDGF
29
(No Transcript)
30
(No Transcript)
31
(No Transcript)
32
vWf
B. Wasowska et al 2001 Transplantation 71, 727.
33
Ig Knock Out Recipients
34
Operating Characteristics of ANA
Immunofluorescence Assays
1320
1160
180
140
Tan et al. Arth Rheum 40 1601-1611, 1997
35
HUMORAL IMMUNITY IN CARDIAC TRANSPLANTS
Cardiac Surgery J. Conte Cardiology D Carter,
EK Kasper, D Judge Immunogenetics D Lucas, MS
Leffell, AA Zachary Bioengineering J Haun, R
Alevriadou Pathology RH Hruban, ER Rodriguez, WM
Baldwin III