AP BiologyName ______________________________________

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AP Biology Name ________________________________
______ Urinalysis Lab Unit 7 Mods.
___________ Date ____________ URINALYSIS Analysi
s of urine (urinalysis) can indicate whether
there is an imbalance of various substances in
the blood. Various components of the blood may
be filtered incorrectly in the kidney. As a
result, a physician may order further diagnostic
tests to determine the extent of the kidney
malfunction. You will have four teacher urine
samples to test in your lab analysis. Procedure
Retrieve safety glasses..turn on and set-up
hot plate for Test 5, Reducing Sugars.  Obtain 5
ml of each of the 4 teacher samples in test
tubes. Be sure to label each. TEST 1 -
Appearance  Note any unusual color, smell, or
cloudiness in the urine sample. Use a dropper to
add samples to the pH paper. Record in the data
table. TEST 2 - pH  Use the pH paper to
determine the correct power of Hydrogen in each
sample. Record in the data table. TEST 3 -
Glucose Use the Chemstrip stick to determine
the amount of glucose in each sample (mg/dl).
Allow 2-3 minutes for the test to be complete.
Record in the data table. TEST 4 - Protein
Use one dropperful of Biurets Reagent to test
for the presence of protein in the urine.
(Remember that the solution should turn purple in
the presence of protein.) Record in the data
table. TEST 5 - Reducing sugars Obtain another
5 ml of each of the 4 teacher samples.  Use one
dropperful of Benedict's Reagent and heat for 5-6
minutes to test for the presence of reducing
sugars (sugars with free aldehyde or ketone
groups). Record in data table. Data Table.
1. Teacher Name Appearance
pH Chemstrip Biuret
Benedict's ___________________________________
_________________________________________________
_________________________________________________
___________________________________ _____________
__________________________________________________
_____________________ ___________________________
__________________________________________________
_______ 1 - Over, please
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Analysis For each of the teacher samples,
identify the possible causative agent for any
abnormal urinalysis test. Use nephron
terminology in your diagnosis. Teacher
Name Urinalysis Diagnosis ______________________
__________________________________________________
_______________ _________________________________
__________________________________________________
____ ____________________________________________
___________________________________________ _____
__________________________________________________
________________________________ For the
diagram below, identify the following name of
structure, nephron process that occurs there,
description of filtrate /urine (hyperosmotic,
hypoosmotic, isoosmotic) following these
processes and a description of blood after
filtration, secretion, and reabsorption in Data
Table 2. afferent arteriole loop of
Henle efferent arteriole venule  glomerulus
distal convoluted tubule  collecting
duct proximal convoluted tubule Bowman's
capsule  peritubular capillaries

10 2-Over, please
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Data Table 2.
3-