Arabidopsis ecotypes infected with Fusarium oxysporum WT and a fosnf1 mutant - PowerPoint PPT Presentation

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Arabidopsis ecotypes infected with Fusarium oxysporum WT and a fosnf1 mutant

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H2O. WT. fosnf1. Cape Verde Col-0 EST. Arabidopsis ecotypes infected with Fusarium oxysporum (WT) ... Agrobacterium tumefaciens-Mediated Transformation of Fungi ... – PowerPoint PPT presentation

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Title: Arabidopsis ecotypes infected with Fusarium oxysporum WT and a fosnf1 mutant


1
Arabidopsis ecotypes infected with Fusarium
oxysporum (WT) and a fosnf1 mutant
Cape Verde Col-0
EST
H2O
WT
fosnf1
2
GFP-labeled F. oxysporum colonizing the root
xylem of Arabidopsis
3
Transformants (10-100 / plate)
Phenotype characterization
Agrobacterium tumefaciens-Mediated Transformation
of Fungi
4
OCCURRENCE OF AVR-Pita IN THE USA FIELD ISOLATES
OF M. grisea
A
B
C
D
E
F
G
H
BM5-11
BM1-24
75A11
CA205
CA305
75A10
75A49
LO1-4
82A1
LO3-8
A598
85M2
A264
A119
85L2
85A7
75A1
A347
74T3
49D
793
D1
S1
Distribution pattern of the AVR-Pita gene family
among rice isolates of Magnaporthe grisea
Probe Fs
5
PCR of marker genes
PCR of marker genes
DNA extraction from pure cultures of plant
pathogens
DNA extraction from diseased plant or cultured
isolate
Sequencing/Genotyping of marker genes
Pathogen Database
Database searching
Database building
Known phenotypes of pathogen isolates
Risk assessment implementation
of control/eradication measures
Schematic diagram of the plant pathogen database
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