Deconvolution, Visualization and

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• Deconvolution, Visualization and
• Analysis Software for
• Widefield and Confocal Microscopy

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• Media Cybernetics is committed to delivering the
  most advanced algorithms available on the market
  today by the cutting edge research to develop the
  most advanced algorithms for tomorrow.

Media Cybernetics provides uncompromised results
with faster performance than ever before.
The best just keeps getting better and faster
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Image Formation
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What are the problems?

• 2D Case Point sources of light in the sample
  form Airy discs in the image.
• 3D Case Point sources of light in the sample
  form Point Spread Functions (PSFs) in the image.
• Cameras introduce electronic and thermal noise.
• Low light levels introduce shot noise or counting
  noise.

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The Point Spread of a Microscope

• Every microscope has a different point spread
  function.
• Every element in the optical path affects the
  point spread function
• Scope
• Objective
• Coverslip
• Sample

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PSF and Modality

• Confocal and Widefield PSFs

Confocal
Widefield
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Where does the blur come from?
A blurry image is the convolution of an object
with the point spread function of the microscope.
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How do you get rid of it?
Deconvolution
Deconvolution is used to separate the original
object and the point spread from the collected
data (2D or 3D image).
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Why Media Cybernetics

• Established technological focus in deconvolution
  over 17 years of dedicated research
• Breadth of algorithms that are well known and
  accepted world-wide optimized for maximum
  robustness
• Media Cybernetics stands behind all of its
  products and provides world class education and
  support.
• Committed focus to deconvolution, visualization
  and analysis RD ensures customer access to the
  latest technologies for today and tomorrow.

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Media CyberneticsOffering complete
post-processing solutions

• Image improvement, restoration, visualization and
  analysis that facilitates more accurate and
  compelling results.
• Media Cybernetics creates the tools to help you
  do the science.
• AQI Product Line
• AutoDeblur (2D, Silver, Gold)
• AutoVisualize (with improved 3D-Viewer)
• AutoAnalyze (FRET, Ratio, Object
  Counting/Tracking, Colocalization)
• Tools (automatic, manual and channel-channel
  alignment)
• Image-Pro Product Line
• Image-Pro Plus and Image-Pro Plug-In module
  bundles
• Express
• Analyzer