A Biology and Engineering Cooperative Project

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A Biology and Engineering Cooperative Project

• James W. Stevens
• M. Karen Newell
• University of Colorado at Colorado Springs
• 30 April 2005

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general background

• connection between cellular metabolic pathways
  and immune system response
• surface signals tied to mitochondrial activity
  and fuel

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cooperative effort

• biology
• science driver
• cell cultures
• growing
• conditions
• cell counting and physical preparation

• engineering
• data acquisition
• temperature measurements
• data reduction
• data smoothing

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background-this project

• 2 mouse leukemia lines
• L1210 not drug resistant
• L1210/ddp drug resistant
• cultured to 106 to 108 cells
• stressed with bleach
• measurable, repeatable differences
• different culture conditions, metabolic states

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data analysis

• first law of thermodynamics

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data smoothing

• least square curve fit
• minimize

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summary

• leverage complementary capacities specific to
  people and project
• involve students
• plunge into a new area

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other cooperative efforts at UCCS

• biomechanics biology, mechanical engineering
• orthopedic mechanics MERIC, biology, mechanical
  engineering
• feedback controls MAE, biology
• microgravity MAE
• assistive technologies MATH, biology