RNA Processing

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RNA Processing

• Reading
• Annual Review of
• Biochemistry
• 72291-336

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RNA Processing

• RNA Polymerases
• RNA Polymerase I rRNA
• RNA Polymerase II mRNA, snRNAs
• RNA Polymerase III tRNA, U6 snRNA,
• 5S rRNA

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RNA Processing

• RNAs
• pre-mRNA gRNA
• mRNA pRNA
• snRNA tmRNA
• tRNA siRNA
• rRNA miRNA

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RNA Processing

• Capping
• Packaging
• Polyadenylation
• Splicing
• Editing
• Base Modification
• Degradation/Stability
• Transport

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RNA Cap Structures
mRNA 7meG
snRNA 2,2,7meG
5-5 Bond
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Cap Function
Translation Splicing Polyadenylation Transport Sta
bility
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Splicing snRNAs
RNA Abundance Cap
U1 1X106 m3GpppA U2 5X105 m3GpppA U4 2X105 m
3GpppA U5 4X105 m3GpppA U6 4X105 mpppG U11
(AT-AC) 1X104 m3GpppA U12 (AT-AC)
5X103 m3GpppA U4atac (AT-AC) 2X103 m3GpppA U6a
tac (AT-AC) 2X103 mpppG
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DNA Template
Transcription
Radiolabeled Pre-mRNA
Nuclear Extract
In Vitro Splicing
300C, ATP
Native Gel Electrophoresis
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DNA Template
Transcription
Radiolabeled Pre-mRNA
Nuclear Extract
In Vitro Splicing
300C, ATP
Phenol Extract Precipitate RNAs
Denaturing Gel
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GT-AG vs. AT-AC Splicing

• Consensus Sequences
• 5 SS Branch Points 3 SS
• gtaagt ynyuray yag
• atatccty tccttray yac

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hnRNP Proteins
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