GEM Presentation

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GEM Presentation
Germplasm Enhancement of Maize

• GEM Team
• USDA-ARS NCRPIS GEM
• - December 8, 2004 -

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Quality Trait Research

• Susan Duvick
• Corn Quality Traits Lab Manager

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Wet Chemistry Laboratory
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Personnel

• Susan Duvick, Laboratory Manager (NCRPIS)
• Kaily Chang, Research Associate (CICGRU)
• Oswaldo Taboada-Gaytan, Graduate Student (CICGRU)
• Joe Van Gorp, Undergraduate Intern (CICGRU)
• Carli Petersen, Undergraduate Intern (NCRPIS)
• Cooperating scientists and project members

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Corn Quality Traits Laboratory

• Primary Functions
• Germplasm evaluation for quality traits
• Method development for new quality traits
• Research leading to high quality corn germplasm
• CRIS Projects
• Germplasm enhancement of maize project (GEM)
  (Mike Blanco)
• Enhancing corn germplasm for quality traits
  (Linda Pollak)
• Breeding high-quality corn for sustainable,
  low-input farming systems (Linda Pollak)
• Quantitative genetics and grain quality of corn
  (Paul Scott and Jode Edwards)

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Current Analytical Methods

• Whole grain composition (POS) by NIR using sample
  sizes from 1 kernel to 1 pound
• Specific density by NIR using sample sizes from 1
  ear to 1 pound
• Extractable starch by NIR using sample sizes from
  1 ear to 1 pound
• Starch thermal properties by DSC using sample
  sizes from 1 kernel to bulk starch samples
• Starch viscosity properties by RVA using sample
  sizes of starch from at least 10 grams of corn
• Fatty acids by GC from single kernels
• Wet milling by both bench top single kernels and
  100g using a starch table

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NIR Whole Grain Composition
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NIR Sample Sizes
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GEM Results-Composition

• Protein gt 15
• Oil gt 4
• Starch gt 71
• B73 values 14 protein, 3.5 oil, 70 starch

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Starch Extraction
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Bench Top Wet Milling
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100g Wet Milling
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Starch Thermal Analysis
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DSC Curve
CH05015N1204-57-1-B-B
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GEM Results-Starch Quality

• ToG lt 62oC
• TrG lt 5oC
• R gt 60
• Commercial corn starch ToG, TrG, R

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Field Day 2004
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Recommended GEM Lines for 2004
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New Methodology

• ETOH by bench top laboratory procedure
• Food applications of oils with altered fatty
  acids
• Calculating breeding parameters for quality
  traits
• Modeling from our existing data to predict
  resistant starch
• Thermoelectric conductivity and electroactive
  transport
• Plasticizing starches

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GEM Presentation
Germplasm Enhancement of Maize

• The End