Sophisticated Gene Synthesis & Cloning is Easy at Creative Biogene - PowerPoint PPT Presentation

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Sophisticated Gene Synthesis & Cloning is Easy at Creative Biogene

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At Creative Biogene, we can synthesize and clone any DNA sequences, even complex, GC-rich, repeated, or long genes, into prokaryotic, eukaryotic or viral vectors. – PowerPoint PPT presentation

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Title: Sophisticated Gene Synthesis & Cloning is Easy at Creative Biogene


1
Sophisticated Gene Synthesis Cloning Is Easy at
Creative Biogene
2
The Traditional Cloning
  • For a traditional cloning process, it is
    generally thought to be lengthy and complex for
    eight reasons, as follows
  • Cloning strategy design
  • Primer Synthesis
  • PCR and restriction enzyme digestion
  • Gel extraction and ligation
  • Transformation and plasmid miniprep
  • Screen by enzymatic digestion
  • Sequencing reaction preparation
  • Sequence assembly analysis

3
Challenges
  • Either to solve those issues or skip to the next
    step, the process can still be challenge due to
    factors, such as a physical template, minor or
    major changes, intensive labor, unpredictable
    results, etc. All those procedures can drain your
    reagents and  precious   time that supposed to be
    used in more refined points.

4
Cloning at Creative Biogene
  • At Creative Biogene, we can synthesize and clone
    any DNA sequences, even complex, GC-rich,
    repeated, or long genes, into prokaryotic,
    eukaryotic or viral vectors.
  • Creative Biogene has accomplished hundreds of
    thousands of gene-synthesis cloning projects
    for scientists over the world.
  • Creative Biogenes gene synthesis and Cloning
    service is flexible to accommodate projects from
    single gene synthesis to whole genome synthesis. 

5
Creative Biogenes Gene Clone Service includes
  • Codon Optimization for any given expression
    system. Synthesis of genes of interest with or
    without tags, and cloning the target sequence
    into a standard vector.Sub-cloning of your
    synthetic gene products into a vector of your
    choice.Amplification of specific cDNA by
    PCR/RT-PCR and cloning into a standard vector or
    a vector of your choice.

6
Other services provided by Creative Biogene
  •   Custom Viral Service  RNAi
    Service   Gene Service  Aptamer
    Service  Plasmid DNA Production
    Service  Stable Cell Line Generation   Oligonu
    cleotide Synthesis Service

7
Contact
  • USA45-1 Ramsey Road, Shirley, NY 11967, USATel
    1-631-626-9181Emailinfo_at_creative-biogene.com
  • United Kingdom St John's Innovation Centre,
    Cowley Road, Cambridge CB4 0WS, UK Tel
    44-207-048-3343 Emailinfo_at_creative-biogene.com
  • Web http//www.creative-biogene.com/Services/Gene
    -Service/Gene-Synthesis-and-cloning-service.html
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