Introduction for Absolute Quantification

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Introduction for Absolute Quantification
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Introduction for Absolute Quantification

• Summary Absolute Quantification, a technique, is
  a targeted quantitative proteomics. This kind of
  technique can express the strong efficacy and is
  being increasingly used for a full range of
  quantitative proteomics research. We will mainly
  introduce the function and advantages of
  technique--Absolute Quantification.
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• Body The technique AQUA method can not only be
  the proteins absolute quantification( AQUA), but
  their modification states. Peptides are combined
  with incorporated stable isotopes, which is
  regarded as the ideal internal standards for the
  mimic native peptides, and this native peptides
  is formed by proteolysis. We also can prepare
  these synthetic peptides by using covalent
  modifications, such as methylation, acelylation,
  phosphorylation. The modification we used are
  identical chemically to naturally happening
  posttranslational modifications. After that, this
  kind of AQUA internal standard peptides are
  utilized to exactly and quantitatively measure
  the absolute levels of proteins and
  post-translationally changed proteins after
  proteolysis with the method of a selected
  reaction monitoring analysis, which happens in a
  tandem mass spectrometer.
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• The development in biological mass spectrometry
  have made the advances of numerous methods for
  the large-scale quantification of protein
  expression levels, which all are processing in
  the cells. In addition to the measurements of
  protein expression accomplished by differential
  incorporation of stable isotopes in to cellular
  proteins. for the proteomics analysis, the
  absolute quantification is also an wonderful way.
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• The functions of the absolute quantification a
  common process for the post-translational
  modification and the quantification of proteins.
  Absolute quantification can be offered by AQUA
  with employment of synethic peptides, which is
  mixed with stable isotope.
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• There are one base for the absolute
  quantification method, it is the discovery of
  unexpected relationship between the protein
  concentration and MS signal response. The average
  MS signal response, which is for the three most
  intense tryptic peptides per mole of protein, is
  not going to be changed within a coefficient if
  variation of less than 10. This relationship can
  be utilized to estimate a general signal response
  factor. If given an internal standard. The
  general signal response factor was expressed to
  be similar to all protein testing. However the
  isotope way build only relative quantification of
  proteins, which are expressed. The absolute
  quantification(AQUA) method can offer for precise
  determination of protein expression and the
  levels of post-translational modification. This
  AQUA method depends on the use of a synthetic
  internal level peptide, which is introduced
  during digestion, as a known concentration to the
  cell lysates. When we analyze the proteolyzed
  with a selected reaction monitoring(SRM)
  experiment, which is processed in a tandem mass
  spectrometer. It is may cause the direct
  detection and the native peptides quantification
  and isotope, which is labeled with AQUA internal
  standard peptide.
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• Because of the sensitivity and simplicity of the
  method, added with the tandem mass spectrometers
  widespread availability, AQUA method becomes a
  most useful process for us to measure the
  proteins levels and post-translational
  modifications directly from cell lysates.
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• About the author Creative Proteomcis, the
  proteomcis division of CD Inc, has the whole
  services in the drug development field. We are
  specializing in the hard-to-analyze samples, that
  is because of the scientists, who have many
  experience in this field. We also have maintained
  the close relationship with our partners,
  providing the lowest cost for these industry.
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• Links
• http//www.creative-proteomics.com/Services/Absolu
  te-quantification-AQUA.htm
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• http//www.creative-proteomcis.com
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