The Difference between Polyclonal Antibody and Monoclonal Antibody

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The Difference between Polyclonal Antibody and
Monoclonal Antibody
It is known that antibody is a large Y globulin
secreted by Effector B cell and antibody can
identify and neutralize the virus and bacteria.
Thus antibody plays a vital role in life
science.As the research of antibody goes,
antibodies can be made by human and it becomes
various. It is always divided into two parts
polyclonal antibody and monoclonal antibody. The
difference between polyconal antibody and
monoclonal antibody will be presented in this
blog. Definition Generally, the antibodies are
made up of several antigen determinants. If the
antibody is stimulated by one kind of antigen
determinant and it is produced by the interaction
of one Effector B cell and antigen, it is the
polyclonal antibody. However, several antigens
stimulate the organism and it would produce
various polyclonal antibody, then these
polyclonal antibodies will mix up and they become
monoclonal antibodies. Beside of the variety of
antigen determinants, the same antigen
determinants also can stimulate organism produce
five different kinds of antibodies including IgE
?IgD?IgA?IgG and IgM.
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In addition, monoclonal antibody is stimulated by
heterologous antigens and then organism produces
immune reaction. It is produced by organism
plasma cells. However, polyclonal antibody is
cloned by one antibody which only has reaction to
the epitope of a specific antigen. Application
and uniques Polyclonal antibody The main merit
of polyclonal antibody is that use antigen with
general purity can get a large number of
antibodies with high specificity and uniformity.
Because of this advantage, it can be applied to
immunological analysis which is characterized by
labeled antibodies such as IRM and ELISA.
Moreover, it is also beneficial to inside
radioimmunoimaging ,immune -directed therapy,
protein purification and medical diagnostic
testing reagents.
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Monoclonal antibody monoclonal antibody has a
wider application than polyclonal antibody for
its advantages. For example, it can recognize
quantities of epitopes it can causes
precipitation reaction preparation time is short
and its cost is low. Those are the basic
difference between polyclonal antibody and
monoclonal antibody. Resource http//www.creat
ive-diagnostics.com/blog/index.php/the-difference-
between-polyclonal-antibody-and-monoclonal-antibod
y/