How to Make Your Own Spore Syringe?

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How to Make Your Own Spore Syringe?
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What is a Spore Syringe?

• A spore syringe is basically a medical syringe
  filled with magic mushroom spores suspended in
  sterile water. They store active spores for long
  periods of time. These syringes are used to
  inoculate substrates to grow your own mushrooms.
• The sterile water suspension also hydrates the
  spores before the important step of inoculating
  the substrate. Spores need to be hydrated for at
  least 24 hours for a higher chance of success.
  Shrooms that are too dry wont be useful at all.

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Supplies to Make a Spore Syringe

• Its not enough to have a clean work space. When
  it comes to growing magic mushrooms and preparing
  a spore syringe, a sterile work space is
  important. Even a small contamination can ruin
  your cultivation. When a harmful organism, such
  as bacteria or mold, soils your colonies, it will
  cause a domino effect and spoil your mushrooms.
  Not taking the right precautions will lead to
  failure, lost investment, and wasted time.
• As much as it is important to work fast when
  working with shrooms, its even more important to
  incorporate a clean technique into your methods.
  Make sure your working area is thoroughly
  cleaned, doesnt have dust, and is in an isolated
  area in your house which is free from flying
  particles. Even dust can damage your young
  colony. Contaminants will compete with the
  mushrooms for nutrients, light, and moisture.
  Eventually, they will harm or kill off your
  mushroom colonies.
• However, be careful. Most antiseptics are
  alcohol-based and flammable. Be extra careful
  when using disinfectants in an area with an open
  flame.

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Supplies to Make a Spore Syringe

• The following supplies are needed
• A sterilized inoculation loop or scalpel
• A pair of sterilized tweezers
• A spore print (depending on the size and
  saturation of the spore print, you can make
  multiple syringes from one spore print)
• Sterile syringe and needle
• A small bottle or flask with a small opening
• If sterile water is not available, you can use a
  pressure cooker to sterilize the water
• Tinfoil ripped to cover the container
• Sterile latex gloves
• A sterile face mask

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Steps in Making a Spore Syringe

• Sterilize the water
• Sterilize the flask or glass bottle
• Sterilize the rest of the equipment
• Fill the water with spores
• Fill the sterile syringes with spores

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1 Sterilize the water

• To produce sterile water, use a pressure cooker.
  The heat and pressure will destroy microbes which
  can contaminate your future grow and make sure
  your spore syringe wont contaminate your
  colonies.

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Sterilize the flask or glass bottle

• Use a flask or a glass bottle with a narrow
  opening to reduce the chances of contamination or
  microbes getting inside it. Pour 30 mL of water
  into it. Use the tin foil to cover the flasks
  opening and place it into the pressure cooker.
• Make sure to close the lid of the pressure cooker
  properly and let it heat up until it has reached
  a pressure of 15 PSI. Maintain this pressure for
  at least 30 minutes then let it cool off
  thoroughly for at least 5 hours. If possible, let
  it cool overnight.
• You now have sterilized water inside a flask. Be
  careful that its not too hot when the spores are
  introduced. Otherwise, they will not survive.

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Sterilize the rest of the equipment

• Now its time to sterilize the rest of your
  equipment.
• If youre reusing syringes and needles, you need
  to sterilize them too. Wrap them in tin foil and
  also put them in the pressure cooker at a
  pressure of 15 PSI for 30 minutes.
• The scalpel and the pair of tweezers are prime
  candidates for sterilization because they can
  also contain microbes on them. Its better to put
  in the extra effort of sterilizing them now than
  risk losing your mushrooms later on.
• Use a Bunsen burner to heat the ends. Apply heat
  until they are glowing red from the heat.
  Afterwards, put them in a stand and make sure the
  ends are not in contact with anything. Let them
  cool off for 30 seconds before using them for the
  next step. Otherwise, your spores will become
  useless.

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Fill the water with spores

• Take the spore print out of its container. Using
  a scalpel that was just sterilized, scrape off
  the spores from the paper and into the sterilized
  flask with the sterile water. If the water still
  seems clear, just continue scraping some more
  spores off. Youll know you have scraped off
  enough spores when you look into the flask and
  they are visibly floating or suspended.
• As soon as you have enough spores in the water,
  close the flask with the tin foil. Make sure it
  stays sterile and uncontaminated.

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Fill the sterile syringes with spores

• Assemble the sterile syringes. Dip the tip of the
  syringe without the needle and pull the plunger
  back to suck in the water along with the spore
  suspensions. After filling a syringe, seal them
  using the needle and its cap.
• Repeat the process until you have filled every
  syringe you have. When the syringes are filled
  with the spore suspension, store them at room
  temperature for 24 hours before using them to
  inoculate the substrate. This will hydrate the
  spores and make substrate inoculation possible.
• If you wont use the syringe right away, you can
  store them inside a refrigerator at a temperature
  of 2 to 4 degrees Centigrade. They have a
  lifespan of around 6 to 12 months. However,
  remember their viability will fade after a couple
  of months. The longer you keep them, the less
  potent they become.

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Fill the sterile syringes with spores

• When storing spore syringes, make sure they are
  labeled with the strain of mushrooms, and the
  date they were prepared. Keep them in an airtight
  Ziploc bag.
• Once you are ready to use your shroom spores,
  inoculate your chosen substrate by inserting the
  needle halfway and pushing the plunger. With the
  mushroom spores syringe prepared through this
  method, you will need only 3mL of spore solution
  to colonize 1 liter of substrate. A 20mL syringe
  is enough to inoculate 6 different substrates.
• After a few days, white patches will start
  growing on places where the spores were injected.
  It may take weeks for the whole substrate to be
  completely colonized. When the fruiting bodies
  appear, harvest them accordingly.

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Fill the sterile syringes with spores

• You can take this a step further and use their
  spore prints to create even more spore prints and
  spore syringes for future use.
• Making your spore syringe and growing your own
  shrooms is easy but it will take time and a lot
  of effort to maintain a sterile working
  environment.
• If you dont have the finesse to grow your own
  magic mushrooms with your own spore syringe, a
  magic truffle grow kit may be the choice for you.
• A magic truffle grow kit from Truffle Magic is
  guaranteed to produce psychedelic truffles in a
  short amount of time through a process that
  requires little to no effort!

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