Protein Quantitation

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Protein Quantitation
Creative BioMart
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By many years of experience in protein
researches, Creative BioMart provides a
comprehensive range of analytical development and
testing services for protein quantitation. Our
testing services provide high-quality analysis
required throughout discovery, development,
clinical trials, and commercialization. Assays
can be transferred, developed, verified,
qualified or validated, and can be run under RD,
GLP or GMP conditions. Professional and strict
documentation and management systems have been
developed to efficiently support all the projects.
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From the services below, our customers could
benefit the complete analytical quantitation
packages, including individual analysis or a full
protein analysis package to fulfill regulatory
requirements.
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Active Concentration Analysis
We offer service on protein activity studies
using the most advanced technologies, such as
xMAP and bDNA based high throughput techniques,
protein array, SPR by Biacore system,
bioluminescence, etc. Below is a short list of
analytic service available in Creative BioMart.
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• Enzyme activity assay, including kinase,
  esterase, phosphatase, dehydrogenase, synthetase,
  etc.
• MMP, adhension panel
• Transcription factor (NF-?B P65)
• Cytokine, chemokine, growth factor
• Promoter methylation array
• ATP determination
• Antioxidation/ free radicals test
• Biochemical metabolism assay
• Tumor markers and disease detection

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Find your own case below

• Cytokines chemokines (6Ckine/CCL2, EGF,
  ENA-78/CXCL5, Eotaxin )
• Cell signaling pathway (Active Caspase 3,
  Akt/PKB, ATF2)
• Brain-derived neurotrophic factors (Cortisol, Apo
  Al, BDNF, Cathepsin D)
• Bone metabolic factors (RANKL, Adiponectin,
  IL-1ß, ACTH, Insulin)
• Skin growth control factors (Cortisol,
  Fibronectin, HAS)
• Apoptosis and sepsis related factors (MIF, PAI-1,
  sFas)
• Immunoglobulin (IgA, IgG1, IgG2, IgG3, IgG4, IgM)
• Tumor biological markers (CA-125, IGF-II, Leptin,
  MIF, Osteopontin)
• Endocrine hormones (ACTH, AGRP, Amylin, BDNF)
• Cardiovascular factors (Adiponectin, Apo Al,
  IFN-?, TNFa)
• Renal toxicity studies (Albumin, Clusterin,
  Cysatin C, Kim-1)
• Metabolic factors (Amylin, AFP, ANGPTL3)

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Standard Concentration Measurement
Several UV and visible spectrophotometers
platforms are widely used in protein analysis.
The Ultraspec series of UV/visible
spectrophotometers, for example, provides modules
for protein determination, enzyme activity
kinetics, and fraction analysis. Platform at
Creative BioMart offers you regular protein
quantitation service including basic absorbance,
transmittance, OD600, and concentration methods
as well as Bradford, BCA, Biuret, and Lowry
protein quantitation methods.
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iTRAQ Multiplex Protein Quantitation
Analysis and database searching of protein
database with LC-MS/MS. Differential protein
expression profiling can be obtained using
multiplex tagging reagents and LC/MS peptide
analysis. The tryptic peptides from each
biological sample are labelled with one of the
tagging reagents, such as iTRAQ or dimethyl
reagents, the peptides are combined, separated
and purified by strong cation exchange
chromatography (SCX) or Hydrophilic Interaction
Chromatography (HILIC) chromatography. The
peptides are then analysed by LC-MS/MS on a QTOF
or Orbitrap mass spectrometer. The MS/MS data are
used for database searching and peptide
identification, and the differential labels are
used for relative quantification of each peptide.
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Advantages

• Thousands of peptides identified and quantified
  in one single analysis Large dynamic range, high
  and low abundant proteins are identified High
  diversity of proteins observed, including acidic,
  basic, high Mw, and hydrophobic membrane
  proteins.
• Proteins from host cells, cell culture media,
  bacteria or virus that has infected of a cell
  culture.
• Degradation products cleaved protein forms,
  truncated protein forms, oxidation and
  deamidation products.

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Thanks for watching! Contact Creative
BioMart Address 45-1 Ramsey Road Shirley, NY
11967, USA Email contact_at_creative-biomart.com W
ebsite http//www.creativebiomart.net