Site-Specific and Custom Labeling - PowerPoint PPT Presentation

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Site-Specific and Custom Labeling

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Creative BioMart is committed to providing advanced tools for protein expression and protein purification. As a leading supplier for reagents in the biotechnology field, we understand the importance of convenient and easy-to-use systems for high level expression and sample purification. We invite you to review our growing range of expression systems resulting from our experience in cloning, overexpression and purification. – PowerPoint PPT presentation

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Title: Site-Specific and Custom Labeling


1
Site-Specific and Custom Labeling
Creative BioMart
2
Creative BioMart is committed to providing
advanced tools for protein expression and protein
purification. As a leading supplier for reagents
in the biotechnology field, we understand the
importance of convenient and easy-to-use systems
for high level expression and sample
purification. We invite you to review our growing
range of expression systems resulting from our
experience in cloning, overexpression and
purification.
3
  • Site-Specific Labeling
  • C-terminal labeling
  • N-terminal labeling
  • Labeling at glycosylation sites

4
Especially biomolecule labeling requires reaction
procedures that can be performed under
physiological conditions (neutral pH, aqueous
solution, ambient temperature) with low reactant
concentrations to ensure non-toxic, low
background labeling at reasonable time scales
while still preserving biological function. Among
the plethora of possible reactions only a few
generally fit the necessary reactivity,
selectivity and biocompatibility criteria.
5
  • Cu-catalyzed Azide-Alkyne reaction
  • Strain-promoted Azide-Alkyne reaction
  • Tetrazine-trans-Cyclooctene Ligation

6
Creative BioMart has extensive experience with
various protein labeling techniques and are
confident that we can provide you with
high-quality labeled reagents for your downstream
applications. Possible problems during labeling
procedures include protein losses due to
precipitation, sample manipulation instability,
inconsistent label-to-protein ratio, incomplete
removal of an unconjugated labeling probe, and
poor protein characterization before and after
labeling. Creative BioMart has new labeling
technologies that had emerged for site-directed
labeling requiring an integration of protein
expression and purification into the labeling
process.
7
Thanks for watching! Contact Creative
BioMart Address 45-1 Ramsey Road Shirley, NY
11967, USA Email contact_at_creative-biomart.com W
ebsite http//www.creativebiomart.net
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