Immunology

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Immunology IMM 334Y/335Y
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Immunology IMM334Y/335Y Co-ordinator M.
Ratcliffe Room MSB 5255 Phone 416-978
6382 email michael.ratcliffe_at_utoronto.ca Instr
uctors M.Julius 416-480 6100x7204 michael.julius
_at_sri.utoronto.ca E. Fish 416-340
5380 en.fish_at_utoronto.ca R. Inman 416-603
5869 robert.inman_at_uhn.on.ca J. Booth 416-480
6100x3266 james.booth_at_sunnybrook.ca G.
Levy 416-340 5166 glfgl2_at_attglobal.net R.
Rottapel 416-946 2233 rottapel_at_uhnres.utoronto.ca

My office hours Mondays 8am 1pm..email for
appointment
Tutor Dr. L. Clemenza liliana.clemenza_at_utoront
o.ca
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334Y and 335Y
ISP students must take 335Y to complete their
programs
ISP students must take Biochem BCH371H this year
as there is a timetable clash with JBI428 if
BCH371H is delayed till next year
If you cant take both BCH371H and JBI428 there
will be substantial problems graduating with a
specialist degree
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Immunology IMM334Y/335Y Where and
When Lectures Thursday, 1000am-1200noon,
Earth Sciences, EC 1050 Tutorials 334Y Monday
, 300pm - 400pm, Mechanical Engineering, MC
102 335Y Monday, 2.00pm 300pm, MSB 4171
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Immunology IMM334Y/335Y Resources Lectures wor
th attending!!!! Tutorials 334Y question and
answer with Dr. L. Clemenza Textbook Immuno
biology (Janeway 7th edition) Website www.immuno
logy.utoronto.ca follow links to undergraduate
program an then to IMM334Y or IMM335Y FAQ
list follow links to FAQ on the
website questions will be posted anonymously!!
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Immunology IMM334Y/335Y Exams Term Test
1 November 13th, 2008 25 Term Test
11 February 12th, 2009 25 Final Exam date
and time TBA 50 100 Term test
I covers September 11th to November 6th Term
test II covers November 20th to February 5th
Each 334Y mid term exam will be about 40
multiple choice questions Each 335Y mid-term exam
will be based on short essay questions Time
same time as class (10 am 12 noon) Place Term
Test I Examination Center, 255 McCaul
Street Term Test II Examination Center, 255
McCaul Street Check the website for these
locations and for your room numbers
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Immunology IMM334Y/335Y Final exam covers
everything but biased towards the end of the
course. 334Y About 100 multiple choice
questions 335Y A mix of multiple choice and
essay based questions Time and place TBA Past
papers are posted on the IMM334Y and IMM335Y
websites
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Immunology IMM334Y/335Y Missed Term Test
Policy If a term test is missed due to illness,
then 1. The student must obtain a University of
Toronto Student Medical Certificate, have it
filled out by their physician and submit it to
the Immunology Office within one week of the
missed examination. 2. If the note confirms that
the student was incapacitated on the date of the
test, then the weighting of the students other
graded work (including the final exam) will be
increased by the amount of the missed test. If
the note does not confirm that the student was
incapacitated on the date of the exam, then a
grade of 0 will be assigned for the
test. THERE ARE NO MAKE-UP TESTS
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Immunology IMM334Y/335Y Past Papers The
last 3 years of past exam papers for 334Y are
available on the website. Follow links to
IMM334Y. The last 3 years of past exams papers
for 335Y are available on the website. Follow
links to IMM335Y. Deferred Exam Students who
miss the final exam for a valid reason may
petition to the Faculty of Arts and Science to
write the deferred exam. The format of this exam
will be written answer and is cumulative.
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Immunology IMM334Y/335Y Last Day to Drop Y
Courses February 16th, 2009.
Last Day to switch from IMM335Y to
IMM334Y October 31st, 2008. Note that this
switch is not an option for ISP students
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Immunology IMM334Y/335Y Section 1 setting the
stage- molecules and development Sept.
11 Course Business/Introduction Overview MR
18 Cells and tissues of the hematopoietic
system 25 Immunoglobulin structure
and function Oct. 2 Generation of
antibody diversity 9 B cell
development 16 Signal
transduction in lymphoid cells
23 MHC, Antigen processing JB 30
presentation, T cell development Nov.
6 NK cell biology Nov. 13 Term Test 1
(covers Sept. 11, 2008-Nov. 6, 2008 inclusive)
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Immunology IMM334Y/335Y Section 2 cell
and molecular interactions in immune
responses Nov. 20 Clonal selection MJ
27 Alloreactivity generation of MHC
restricted T cell repertoire P1-gt F1
chimeras Dec. 4 Immune response genes and
holes in the T cell repertoire P1 -gt
F1 chimeras and F1 -gt P1 chimeras Jan. 8 T
dependent B cell activation and the
bystander B cell response
15 Requirements for MHC restricted T-B inter-
actions and the state of B cell
activation Jan. 22 Cytokine biology I EF
29 Cytokine biology II Feb. 5
Cytokine biology III
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Immunology IMM334Y/335Y Section 3
manifestations of the immune response Feb. 12 Ter
m Test II Covers November 22nd 2007 to Feb. 7th
2008 Reading week (Feb 18-22nd) Feb.
26 Innate immunity and complement- RI alterna
tive pathway Mar. 5 Complement classical
pathway 12 Hypersensitivity Types I-IV MR
19 Lupus a paradigm of Systemic
Autoimmunity RR 26 Immunopathogenesis of
viral GL April 2 and bacterial infections
9 Mechanisms of graft rejection and
tolerance induction Final Exams April
21-May 9
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Immunology IMM334Y/335Y The first
problem Pathogens evolve much faster than
humans so how can the human immune system keep
up?
Universal repertoires The immune system can
potentially recognize anything!
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Immunology IMM334Y/335Y The next problem A
protective repertoire needs more than 1,000,000
different specificities and the human genome
contains less than 50,000 genes.
Somatic generation of diversity New
specificities are constantly created
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Immunology IMM 334Y/335Y Leading to another
problem If a universal repertoire is generated
somatically then why would it not react against
self?
It does.but
Tolerance and autoreactivity
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Immunology IMM334Y/335Y Autoimmune
disease Lupus
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Immunology IMM 334Y/335Y And so on. Since
there are different types of pathogens, do all
immune cells have the same functions?
Functional heterogeneity of immune
cells Different types of cell have different
functions
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Origins of Immunology
Jenner and smallpox
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Historical background
Jenner 1796 smallpox
Metchnikoff 1900 Phagocytes
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The score so far Number of infectious
diseases many tens of thousands Diseases
eradicated
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Why only one disease eradicated? 1. Potent
vaccine 2. No animal pool
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Alternative to eradication 1. Control of symptoms
2. Vaccination of each generation but
3. Potential for viral escape therefore
4. Constant battle for new vaccines
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Cells and tissues of the immune system- bone
marrow
HSC- Self renewing
Pluripotent-gives rise to many different types
of cell
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Cells and tissues of the immune system - blood
Common lymphoid progenitor
Granulocyte/macrophage progenitor
Erythro-blast
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Cells and tissues of the immune system - tissues
B cells
T cells
NK cells
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Megakaryocytes and erthroblasts Megakaryocytes
produce platelets that are important in blood
clotting / wound repair
Erthroblasts rapidly dividing source of Red
Blood Cells
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Myeloid cells
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Myeloid cells Neutrophil Stained with neutral
dyes Major granulocyte population (gt90)
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Myeloid cells Eosinophil stains with eosin 2-5
granulocytes weakly phagocytic secreted contents
of granules toxic to parasites
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Myeloid cells Basophil stain with basic
dyes rarest of granulocytes (0.2) granules
contain reactive species eg. histamine
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Myeloid cells Mast cells tissue
bound responsible for local allergic
reactions protection against parasites
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Mast cell degranulation
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Myeloid cells Macrophage tissue bound derived
from monocytes in the blood important in innate
and adaptive immunity
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Myeloid cells Dendritic cells Found in tissues,
can pick up antigen and transport it to secondary
lymphoid organs. Important role in antigen
presentation.
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Lymphocyte small lymphocyte non-dividing in
blood small and boring, with no obvious function
(Gowans)
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Lymphocyte activation
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Natural killer cells (NK cells)
non-B/non-T lymphocytes null cells large
granular lymphocytes Developmentally related to
T lymphocytes
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Developmental lineages Developmental
checkpoints progressive restriction
Regulation microenvironment cytokines
(usually) soluble mediators
Clinical Importance reconstitution of cell
populations stem cell therapy gene therapy