Title: BIBC 102
1BIBC 102 Summer 2005 Lecture 4 August 8, 2005
2Fig. 13.9
3FORMATION OF ATP IN LIVING CELLS
1. Glycolysis 2. Oxidative phosphorylation in
mitochondria 3. Oxidative phosphorylation in
chloroplasts
4BIOLOGICAL WORK
1. Activation of metabolism 2. Transport of
metabolites in and out of cells 3. Biosynthesis
of macromolecules DNA, RNA, protein,
polysaccharides 4. electrical signaling (nerve
cells) 5. Motility and force generation 6.
Regulatory mechanisms
5Chapter 15 in Lehninger Glycolysis Catabolism
of Hexoses
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7VV 16.5
8acid-base catalysis in the isomerase reaction
9VV 16.6
10formation of a Schiff base as a covalent
intermediate in the aldolase reaction
11VV 16.9
12VV 16.10
13LNC 15.7
14LNC 15.8
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17THIAMINE PYROPHOSPHATE
VV 16.28
18Metabolic flux and its control
19LNC 15.16
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21Phosphofructokinase 1
regulatory site
active site
active site
regulatory site
(only two of four subunits shown)
LNC 15.18a
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24Other sugars
- Fructose
- Galactose
- Mannose
pp. 534-537 (Section 14.2) Feeder Pathways for
Glycolysis
25Other Common Sugars
Monosaccharides fructose galactose mannose
Disaccharides sucrose Glu-Fru lactose Gal-Glu
maltose Glu-Glu trehalose Glu-Glu
26Fig. 9.12 in LNC
27Disaccharides
Galactose
Glucose
Figure 7-12
28Glucose
Fructose
Figure 7-12
29Glucose
Glucose
Figure 7-12
30LNC 15.11
31Lactose H2O D-galactose
D-glucoseSucrose H2O
D-fructose D-glucoseTrehalose H2O
2 D-glucose
Enzymes on surface of intestinal epithelial cells
Lactase
Sucrase
Trehalase
p. 535
32Lactose IntoleranceOMIMOnline Mendelian
Inheritance in Man
33Galactose to Glucose 1-P
Figure 14-11
34Figure 14-11
35p. 565
36Figure 14-11
37NADH H
NAD
Figure 14-11, p. 537
38Enzymes required for galactose metabolism
kinase
Galactose Galactose 1-P
UDP-Glu
ATP ADP
Uridyl transferase
epimerase
UDP-Gal
Glucose 1-P
phosphoglucomutase
Glucose 6-P
39GLYCOGEN METABOLISM
LNC Chapter 15
GLYCOGEN SYNTHESIS
LNC Chapter 20
40Chapter 15 pp. 560-570(some figures from
Chapter 7) in Lehninger 4th ed.
41Catabolic PathwaysGlycogen ? Glu-6-P
(glycogenolysis)Glu-6-P ? Pyruvate
(glycolysis)Anabolic PathwaysPyruvate ? Glu
(gluconeogenesis)Glu ? Glycogen (glycogenesis)
42Figure 7-14b
433 enzymes of glycogen breakdown(Glycogenolysis)
- glycogen phosphorylase
- glycogen debranching enzyme
- phosphoglucomutase
44End of lecture 4, August 8, 2005