Virology

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Virology Nipah virus
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Family Paramyxoviridae
Subfamily Paramyxovirinae
Genus Henipaviruses
Nipah virus
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Classification
Nipah virus is an enveloped, nonsegmented,negative
-stranded RNA virus that is a classified member
of the family Paramyxoviridae
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????????? Nipah virus

• ???????????????????????????????? vero E6 cells
  ??? human brain
• negative-stranded RNA viruses
• Negative stain electron microscopy (EM)
• ?? helical nucleocapsids ?????????????
  filamentous nucleocapsids ??????????????? 1900
  nm ????????? envelope ??????????????? roughly
  spherical
• ?????????????????? herringbone
• ??????????? pleomorphic virus particles
  ,extracellular virions ??????????????? 500 nm in
  diameter (range, 1801900 nm).
• ?????????????????????????????? multinucleation,
  intracytoplasmic nucleocapsid inclusions (NCIs)
  ??? long cytoplasmic tubules ????????? 3045 nm
• ???????????? inclusion formed ????????????????????
  ????? ???????????????????? transcription

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Nipah Virus. Electron Micrograph of an infected
Vero cell showing viral particle 200 - 300 nm in
size and its morphogenesis at the cell membrane.
(Photo courtesy of Prof. Lam Sai Kit)
Nipah virus electron micrograph Image courtesy of
C.S. Goldsmith and P.E. Rollin (CDC), and K.B.
Chua (Malaysia).
Nipah virus syncytium with nuclei
characteristically forming a ring around the
periphery of the multinucleated cell(in this case
with the cytoplasm stained to demonstrate Nipah
virus antigens)
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Budding particles. (A) Nipah virus nucleocapsids
(arrow) become tightly aligned along the plasma
membrane as particle prepares to bud. (B) Rigid
positioning of nucleocapsids sometimes formed
fingerprint-like structures. Bars, 100 nm.
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Pulmonary pathology and viral immunolocalization
in Nipah virus infection. A Focal alveolar
fibrinoid necrosis and inflammation. B These
necrotic lesions show prominent immunostaining of
viral antigens. C Multinucleated giant cell with
nuclear inclusions in the alveolar space. D
Viral nature of giant cells as evidenced by
immunostaining for Nipah viral antigens. E and F
Nipah viral antigens in tunica media of small
arteries. G and H Rare instance of
immunostaining of bronchiolar epithelium. HE
stain, A and C immunoalkaline phosphatase with
napthol fast red substrate and hematoxylin
counterstain, B, D, E, G, H. Original
magnifications x100 (A) x50 (B, E, G) x158 (C,
D, F, H).
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