Title: Questions for Chapter 17 and 18:
1Questions for Chapter 17 and 18 17 4, 7,
10, 13 18 2 3
2Comparing Genomes
What are the major genomic differences between
prokaryotes and eukaryotes?
Size E. coli - 4.64 x 106 bps for 4,397
genes S. cerevisiae - 12 x 106 bps for 6,548
genes H. sapiens - 3.3 x 109 bps for
30-35,000 genes
Organization prokaryotes - tend to have 1
circular chromosome - many operons - no
introns, and little repetitive DNA eukaryotes
- multiple linear chromosomes - few operons,
some genes within genes - introns common,
repetitive DNA common
3Comparative Organization of DNA
4Highly Repetitive DNA
- Satellite DNA
- highly repetitive DNA found in genome -
pericentric - VNTRs used for DNA finger printing
- Centromere DNA
- highly conserved DNA maintains
chromosome structure - Telomere DNA
- GGGGTTA in Tetrahymena and GGGATT in
Humans - highly conserved
etc
5Transposon-Derived Repeats
Most human repeat sequence is derived from
transposable elements
6Repetitive Transposed Sequences
Sequence that can move about the genome
Short Interspersed Elements (SINES) less than
500 bp but 1,500,000 copies in the human genome
best characterized is the Alu family
Long Interspersed Elements (LINES)
bigger than SINES best example is
the L1 family 6400 bp in length and present up
to 100,000 copies per genome LTR
retrotransposons retrovirus-like elements
DNA transposons
7Chromatin Organization
What do you get when you put all of these
components together?
8Chromosomes
G-banding
9Evolution of Genomes
?
E. Coli 4.64 Mbps 4,397 genes
H. sapien chromosome 22 - 33.4 Mbps
545 genes 134 pseudogenes
10Evolution of Genomes
11Evolution of Genomes
Genomic duplication and gene duplication
12http//www.ornl.gov/hgmis/publicat/primer/intro.ht
ml
http//www.nature.com/genomics/human/papers/articl
es.html
13Types of Mapping
- Genetic linkage map
- linear description of markers/genes on a given
chromosome with markers closer being inherited
together more often. - Physical Map
- The physical location on the chromosome - two
types - Cytogenetic and physical
14Comparison of Genetic Maps
Gene or Marker
Gene or Marker
Genetic Linkage Map
Restrction Fragments
Contig Library
DNA Sequence
15Linkage Mapping
A genetic linkage map shows the relative
locations of specific genes and DNA markers along
the chromosome. Any inherited physical or
molecular characteristic that differs among
individuals and is easily detectable in the
laboratory is a potential genetic marker.
16Cytogenetic Mapping
- Cytogenetic map or a physical map
- Several levels of physical mapping
- -Chromosome, chromosome arm, band specific
- region within an arm, or a certain
stretch of bases
Karyotype
In situ hybridization
17Macrorestriction maps Top-down mapping
- A single chromosome is cut (with rare-cutter
restriction enzymes) into large pieces, which are
ordered and subdivided. - The smaller pieces are then mapped further.
The resulting macro-restriction maps depict the
order of and distance between sites at which
rare-cutter enzymes cleave.
18Contig Maps Bottom-up Mapping
- The bottom-up approach involves cutting the
chromosome into small pieces, each of which is
cloned and ordered. The ordered fragments form
contiguous DNA blocks (contigs).
- Contig maps consist of a linked library of
small overlapping clones from a complete
chromosomal segment
19Advantages of Contig Maps
- An advantage of this approach is the
accessibility of these stable clones to other
researchers. - Useful for finding genes localized to a small
area (under 2 Mb). - Technological improvements now make possible
the cloning of large DNA pieces, using
artificially constructed chromosome vectors that
carry DNA fragments (BACS, YACS).
20Ultimate Map
DNA sequence is the ultimate map!
21Uses for Gene Mapping
- Find genes (quantitative trait loci) that are
associated with traits of economic importance. - Use genetic markers for marker assisted
selection. - Discover genes causing major physiological
defects. - Develop comparative maps.
22Comparative Genome Mapping
synteny on the same chromosome