Optical Biomedical Imaging and Sensing Andrei Zvyagin azvyaginics'mq'edu'au, Ro' E7A 212 with Xin Zh

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Optical Biomedical Imaging and SensingAndrei
Zvyaginazvyagin_at_ics.mq.edu.au,Ro. E7A 212with
Xin Zhao Centre for Lasers and Applications,
Department of Physics
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Luminescent nanodiamonds for intracellular
imaging

• A biomolecule tagged with luminophore becomes
  visible in the cell
• We develop luminescent nanodiamonds for tagging
  biomolecules, e.g. proteins, DNA
• They are bright, small, non-toxic, and very
  photostable!

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Imaging of individual
nanoparticles and macromolecules

• Imaging resolution is half the wavelength of
  light, but the detection sensitivity of
  individual particles is unlimited.
• We develop a very sensitive microscope to detect
  individual macromolecules, e.g. large proteins,
  and nanoparticles.
• We have detected diamond nanocrystals of 50 nm
  in size.

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Multiphoton microscopy for imaging of live
cells and tissue

• Multiphoton microscopy is an new imaging method,
  which enables imaging of live tissue on the
  subcellular level.
• For example, both sun-screen nanoparticles (zinc
  oxide) and skin architecture are imaged with
  exquisite clarity and resolution (see Image).
• Using this method, we study nanoparticle
  penetration through skin, and regeneration of
  cartilage tissue.