Detecting Aluminum in Drinking Water

1
Detecting Aluminum in Drinking Water

• Erica Sandoval
• Dr. Henry Altmiller

2
Hypothesis Goals

• Fluorescence Method

• Low levels of Aluminum in drinking water can be
  detected using a Photometric technique and GC-MS.

• GC-MS

3
Methodology

• O-Phenanthroline (15mM) and Be2 (0.1M)
• Sodium Acetate Buffer Solution
• Lumogallion
• Heated
• Triton
• Measure Fluorescence

4
Problem

• Detecting 2x10-3 M

• Needed to Detect 7.413x10-7 M

5
Variables Changed

• Sodium Acetate Buffer

• Lumogallion

• Triton

• Time

• Temperature

6
Neat vs. Diluted

• Triton 1 v/v

• Triton

7
Sodium Acetate Buffer

• 100uL pH4.03

• 200uL pH4.49

• 300uL pH4.97

8
Time

• 30 minutes
• 45 minutes
• 60 minutes

9
Temperature

• 25oC

• 4oC

10
Lumogallion

• 100 uL 2.85x10-6M

• 200 uL 5.67x10-6M

• 300 uL 8.46x10-6M

11
Optimum Conditions Found

• Excess lumogallion
• Neat Triton
• 1 hour
• pH 5
• 4oC

12
Why Fluorescent Intensity Improves

• More time to form complex

• All Aluminum Chelated

• Less Collisions

• More Viscous

13
Testing 7.413x10-7M Solution

• Different color

• Less Fluorescent Intensity

14
What About the Instrument?

• Slit Width

• Excitation
• Emission

15
Accomplished

• Learned Instrument

• Found Optimum Conditions

16
Conclusion

• Test Drinking Water

• Different Water Source

• Begin GC-MS Technique