Powerpoint template for scientific posters Swarthmore College

1
Place your title here, try not to make it
too wordy or run over more than two lines as
people will loose interest
David Chandler, Professor Mike Garlepp1 and
Assoc/Prof Erik Helmerhorst2 (main supervisor
always goes last) Institute, University,
School Address of your Institute or University
Materials and Methods
Introduction Put a concise introductory
statement here ensure you finish with the
significance of your research so that is at the
fore of the readers mind when they move onto the
rest of your poster. Make sure it is written for
lay people, even if presenting at a conference
for people in your field. AND REMEMBER this
is the part of the poster that needs to catch the
readers interest, so make it to the point and
make it relevant to your target audience if it
will help solve world hunger, say so
!

• Conclusions
• Ensure that your conclusions address your
  results. If any of your results dont contribute
  to one of your conclusions, then remove the
  results.

Results

• TOP10 E.coli clones 1, 2 and 7 contained a
  single, correctly orientated sRAGE insert in the
  pIB vector.

1. Construction of human sRAGE insect
expression vector
Figure 2. Construction of human sRAGE insect
expression vector.
Use bright colours with good contrasts
Figure 4. Whole cell PCR screening of E.coli
clones transformed with pIB-sRAGE
construct. Positive results are indicated by the
presence of a PCR product approximately 700bp in
size.
Figure 5. PCR of plasmid purified from clones
that tested positive for correctly oriented
pIB-sRAGE insert. Lanes 1 and 3 confirm that
sRAGE is orientated correctly. Lanes 2 and 4
indicate that a single sRAGE insert ligated into
the vector.
Future Directions Let people know where your
research is heading, they may have insight into
it or even wish to collaborate.

• Sf21 Cells transfected with 2µg of pIB-sRAGE
  plasmid and the recommended concentration of
  cellfectamine, produced the highest levels of
  sRAGE expression.

2. Transfection of Spodoptera Frugiperda 21
cells
Figure 5. Western blot of cell media 48hrs
post-transfection of Sf21 cells with varying
amounts of pIB-sRAGE plasmid and
cellfectamine. Levels of sRAGE in the conditioned
media of individual cells, are indicated by
their relative band intensities.
3. Purification of sRAGE from conditioned media

Try and keep your methods simplified not too
much detail, only the essential information.
Acknowledgements The receptor for advanced
glycation end products (RAGE) is a member of the
immunoglobulin super family that has been

• Sf21 Cells transfected with 2µg of pIB-sRAGE
  plasmid and the recommended concentration of
  cellfectamine, produced the highest levels of
  sRAGE expression.

References Make the font for these very small so
that they dont occupy too much space. If people
really want to know a reference, they will step
right upto the poster to read it or ask you.
Place the aim of the research being displayed in
this poster here. Try and keep it to one
sentence if possible.
Figure 3. Purification of sRAGE from culture
media of pIB-sRAGE transfected Sf21 cells.