Day 4 of Bionanotechnology Chem 140240

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Day 4 of BionanotechnologyChem 140/240
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Hyun, et al. J. Am. Chem. Soc. 2004, 126,
7330-7335
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Hyun, et al. J. Am. Chem. Soc. 2004, 126,
7330-7335
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Hyun, et al. J. Am. Chem. Soc. 2004, 126,
7330-7335
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Hyun, et al. J. Am. Chem. Soc. 2004, 126,
7330-7335
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Photolithography
www.ece.gatech.edu/research/labs/vc/theory/photoli
th.html
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Photolithography
www.ece.gatech.edu/research/labs/vc/theory/photoli
th.html
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Smaller Feature Sizes

• Photoacid Generator
• Diphenyliodonium-9,10-dimethoxyanthracene-2-sulfon
  ate (DIAS)

• GCA 6300 I-Line Wafer Stepper
• UCSB Nanofabrication Facility
• ?max 365 nm

Use i-line stepper to pattern arrays of aldehyde
functionality
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Aminooxy Polymers Modified Proteins
Polymer design
Synthesize polymer that can bind to
ketone-modified proteins and can covalently
attach to the surface
Mn 126,600 PDI 2.57 HEMA 8
P. Mansky, Y. Liu, E. Huang, T. P. Russell and C.
Hawker, Science, 1997, 275, 1458-1460
Protein modification via N-terminal
transamination
Dixon, H.B.F. J. Protein Chem. 1984, 3,
99 Gilmore et al, Angew. Chem. Int. Ed. 2006, 43,
5307
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Pattern Formation by PAG Photolithography
AFM shows features
XPS high resolution N scan 401.3 eV before
400.8 eV after exposure
-Polymer is covalently attached to surface by a
prebake step -a-ketoamide-modified streptavidin
is conjugated
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Micropatterns of Streptavidin
Fluorescent antibodies to visualize Signal to
noise 14 Fluorescence only observed with
transformed SA Fluorescence not observed if
protein preincubated with H2NOCH3
Scale bar 40 µm
Transformed streptavidin binds to surface through
a chemoselective N-terminal oxime bond formation
Christman, Broyer, Tolstyka Maynard, J. Mater.
Chem. 2007, 17, 2021-2027