Lipase

1
Lipase
By T Z
2
Background on Lipase

• Enzyme used for absorption of nutrients in the
  intestines.
• Primary objective break down fats
• Reduces number of fat stored in the body
• Produced in the pancreas, then released to the
  small intestines.
• Can also be found in the stomach and foods.

3
Lipase

• Part of the esterases family of proteins.
• Can work in burning and also storing fat.
• Stimulates lipolysis in fat tissue
• Then triggers beta-oxydation pathway( muslce and
  liver)
• Ketone bodies produced in liver
• Used as alternative fuel for glucose
• Contain sulfhydryl
• Activation of Lipase requires those that contain
  sulfhydryl (SH) in reduced state.
• Ascorbic acid
• Vitamin C
• L-Glutathione
• L-Cysteine.

4
Continued...

• Interfacial Activation
• No productivity in water
• With addition of oil, productivity increases
• Lipase regulated by Sc-IPG-phsophonate inhibitor
• When attached, turns off Lipase

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Primary Sequence

• Primary Sequence
• Displays two different Lipase chains
• Shows what those chains are made up of

Chain A
Chain B
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Primary Structure

• This shows which components are responsible for
  alpha helixes and beta sheets.

Beta Sheets 19 beta sheet (9 strands 35
residues)
Alpha Helixes 39 helical (10 helices 72
residues)
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Secondary Structures

• Secondary pictures (2) include two different
  structures

• Alpha Helixes (folding of backbone)

• Beta Sheets

Alpha and Beta
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Lipase Wire Structure
Back View
Front View
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Tertiary and Quaternary Structures

• Tertiary (3) is a 3D folding of the Secondary
  structure.
• Quaternary (4) is two subunits of Tertiary
  attached together. Lipase does not have a 4
  structure because the subunits are not attached.

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Works Cited

• Bacillus subtilis lipase A. RSCB Protein Data
  Bank. 10 Oct. 2007 lthttp//www.rcsb.org//.do?stru
  ctureId1R4Zgt.
• Lipase. University of Maryland Medical Center.
  11 Oct. 2007 lthttp//www.umm.edu//?articles/.htmgt.
  
• Lipase Enzyme and Lipolysis, Important To
  Breal. Vaxa. 10 Oct. 2007 lthttp//vaxa.com/.cfmgt
  .
• Lipase Information Page. Zest for Life. 11
  Oct. 2007 lthttp//www.anyvitamins.com/?lipase-info
  .htmgt.