D' Bailey, D'V' Renouf and E'F' Hounsell' UCL,UK

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http//www.scimedia.com/chem-ed/analytic/ac-meths.
htmspectroscopy
D. Bailey, D.V. Renouf and E.F. Hounsell). UCL,UK

• NMR, MS (MALDI-TOF, GLC-, EI-, ESI-, IT-, FAB-,
  Q4-, MS-MS)

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Complex carbohydrates

• Compound
• Size (mono-, oligo- or polysaccharide),
  complexity/ heterogenicity
• Source, abundancy, stability (alkaline pH usually
  ok)
• Isolation/ purification
• Gel-chromatography, ion-exchange, affinity (Abs,
  lectins)
• Monitoring (polarimetric, RI, UV, PAD,
  isotope/fluor. labelling)
• Enzymatic chemical degradation (PNGase,
  sialidase, hydrazine)
• Analysis/characterization
• Quantitation/ Sugar composition (GLC, GLC-MS,
  HPAEC-PAD)
• Profiling (HPLC, RAAM, CE, methylation analysis)
• Components, linkages, structure, conformation
  (NMR)
• Components (hexose/pentose), sequence
  modifications (MS)
• Molecular modelling (? NOE-restraints ? NMR ?
  MM...)

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Carbohydrate analysis
HPLC
Columns Shodex Asahipak NH2P-50 4E Eluent H2O
/ CH3CN 25 / 75 Flow rate 1.0mL/min Detector
Shodex RI
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Na
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NMR of partially desialylated glycopeptide
DQF-COSY
1D
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