Biochemical pathway and enzyme

1
Biochemical pathway and enzyme
Enzyme a
Enzyme ß
Enzyme ?
S
A
B
C
substrate
Intermediate product
Intermediate product
Final product
Question If enzyme a is nonfunctioning, what
will be absent in the system? What will
accumulate in the system? What if enzyme ß or ?
is nonfunctioning?
2
Ommochrome pathway (brown)
Pteridine pathway (red)
Tryptophan (precursor 1)
Unknown (precursor1)
v, w
bw, w
kynurenine (precursor 2)
tetrahydrobiopterin (precursor2)
cn
hydroxykynurenine (precursor 3)
yellow pterin (sepiaterin product 5)
st
Break down product1
se
red pterin (. product 6)
Break down product2
ommin (end product)
ommatin (end product)
Break down product 3
ry
Break down product 4
3
Paper chromatography for pigments
Pigments are separated and characterized by their
varying affinities to the mobile or stationary
phases of the paper chromatography solvent
components.
Mobile phase organic solvent Stationary phase
chromatography paper
Move up
Red pigments (pteridine pathway) soluble Brown
pigments (ommochrome pathway) insoluble
Stay at the origin